The genome of pest Rhynchophorus ferrugineus reveals gene families important at the plant-beetle interface

Commun Biol. 2020 Jun 24;3(1):323. doi: 10.1038/s42003-020-1060-8.

Abstract

The red palm weevil, Rhynchophorus ferrugineus, infests palm plantations, leading to large financial losses and soil erosion. Pest-host interactions are poorly understood in R. ferrugineus, but the analysis of genetic diversity and pest origins will help advance efforts to eradicate this pest. We sequenced the genome of R. ferrugineus using a combination of paired-end Illumina sequencing (150 bp), Oxford Nanopore long reads, 10X Genomics and synteny analysis to produce an assembly with a scaffold N50 of ~60 Mb. Structural variations showed duplication of detoxifying and insecticide resistance genes (e.g., glutathione S-transferase, P450, Rdl). Furthermore, the evolution of gene families identified those under positive selection including one glycosyl hydrolase (GH16) gene family, which appears to result from horizontal gene transfer. This genome will be a valuable resource to understand insect evolution and behavior and to allow the genetic modification of key genes that will help control this pest.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Evolution
  • Cytochrome P-450 Enzyme System / genetics
  • DNA Transposable Elements
  • Female
  • Gene Transfer, Horizontal
  • Genome, Insect*
  • Insect Proteins / genetics*
  • Male
  • Molecular Sequence Annotation
  • Multigene Family*
  • Plants
  • Population Density
  • Receptors, Odorant / genetics
  • Sex Chromosomes
  • Synteny
  • Weevils / genetics*

Substances

  • DNA Transposable Elements
  • Insect Proteins
  • Receptors, Odorant
  • Cytochrome P-450 Enzyme System