lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis

Qianwen Ouyang; Yanru Cui; Shixin Yang; Wensong Wei; Mingyue Zhang; Jie Zeng; Fei Qu

doi:10.2147/OTT.S241503

lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis

Onco Targets Ther. 2020 Jun 4:13:5033-5046. doi: 10.2147/OTT.S241503. eCollection 2020.

Authors

Qianwen Ouyang¹, Yanru Cui², Shixin Yang¹, Wensong Wei¹, Mingyue Zhang³, Jie Zeng¹, Fei Qu³

Affiliations

¹ Department of Breast Surgery, The Third Hospital of Nanchang, Jiangxi, China Jiangxi Province Key Laboratory for Breast Diseases, Nanchang, Jiangxi 330009, People's Republic of China.
² Department of Physiology, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, People's Republic of China.
³ Department of Pharmacology, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, People's Republic of China.

Abstract

Introduction: The purpose of our research was to evaluate MT1JP in breast cancer.

Material and methods: For clinical purpose, tissues were collected, and a correlation analysis ofMT1JP and miRNA-214 gene expressions was conducted. Using an in vitro study, MDA-MB-231 and MCF-7 cell lines were used as research objects in our research. Colony, flow cytometry, TUNEL, transwell, adhesion and wound healing assay were used to discuss the biological activities of the cells. In an in vivo study, tumor weight and volume were measured, and cell apoptosis was measured by TUNEL assay. The relative mechanism's proteins were evaluated by Western blotting or immunohistochemistry assay.

Results: Compared with adjacent tissues, MT1JP and miRNA-214 gene expressions were significantly different (P<0.001, respectively). By in vitro and in vivo studies, the biological activities of the cells were significantly decreased in MDA-MB-231 and MCF-7 cell lines with MT1JP overexpression. The relative mechanism was correlated with miRNA-214/RUNX3 axis.

Conclusion: The overexpression of MT1JP suppresses the biological activities of breast cancer cells by regulation miRNA-214/RUNX3 axis in vitro and vivo study.

Keywords: MCF-7; MDA-MB-231; MT1JP; invasion; migration; proliferation.

Grants and funding

This study was supported by the National Natural Science Foundation of China (grant/award number 81760527) and Jiangxi University of Traditional Chinese Medicine (grant/award numbers JXSYLXK-ZHYAO123, JXSYLXK-ZHYAO134).