Cellular and serum immune response to dinitrophenol in adult Rana pipiens

Dev Comp Immunol. 1988 Winter;12(1):99-107. doi: 10.1016/0145-305x(88)90028-6.

Abstract

A rabbit anti-Rana anti-DNP antiserum was used in order to estimate changes in frequency of bone marrow (BM) cells containing cytoplasmic anti-DNP antibodies (cId+) for eight weeks following a single injection. We found that (i) cId+ cells increased from 0.2 to 4.3 percent of total mononuclear cells (MNC's) during the first 8 weeks, (ii) BM granulocytes increased in frequency up to week 2, then gradually decreased to week 8, and (iii) serum anti-DNP antibody levels, evaluated by ELISA, increased to week 8. Our observations (i) support those which suggest that Rana BM resembles mammaliam BM as a source of antibody-producing cells, (ii) indicate that specific immune responses in BM may result in a 20-fold increase in the frequency of antigen-related plasma cells among total MNC's, and (iii) suggest that BM B cell clonal expansion, maturation and secretion may result in an increase in specific antibody levels in serum.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibody Formation
  • Antibody-Producing Cells / immunology
  • B-Lymphocytes / immunology
  • Bone Marrow / immunology
  • Dinitrophenols / immunology*
  • Granulocytes / immunology
  • Immune System / physiology*
  • Immunity, Cellular
  • Rana pipiens / immunology*

Substances

  • Dinitrophenols