Alpha-terpineol complexed with beta-cyclodextrin reduces damages caused by periodontitis in rats

J Periodontal Res. 2020 Dec;55(6):877-886. doi: 10.1111/jre.12780. Epub 2020 Jun 25.

Abstract

Background and objective: This study aimed to assess the effectiveness of the treatment with alpha-terpineol (αTPN) complexed with beta-cyclodextrin (βCD) on oral, blood, and hepatic parameters in ligature-induced periodontitis.

Material and methods: Forty female rats were distributed among the following groups: control (vehicle solution), periodontitis (ligature + vehicle solution), 5 mg/kg of αTPN-βCD (ligature), and 25 mg/kg of αTPN-βCD (ligature). Compounds were administered daily via intraperitoneal injection over a 20-day period. Periodontitis was induced with the bilateral insertion of ligatures around the first lower molars of each rat. Oral parameters, as well as blood biomarkers, were measured: histopathological assessment of the hepatic tissue was carried out using light and transmission electron microscopy.

Results: The treatment with αTPN-βCD significantly improved several oral parameters and blood biomarkers in comparison with rats with periodontitis. In addition, the treatment with αTPN-βCD significantly ameliorated the steatosis score and reduced the number of lipid droplets and the amount of foamy cytoplasm in the hepatocytes of rats with periodontitis.

Conclusion: The results obtained suggest that the treatment with αTPN-βCD improves several oral and blood parameters in rats with experimental periodontitis. In addition, hepatic alterations caused by periodontitis were ameliorated in the rats treated with αTPN-βCD.

Keywords: alveolar bone loss; hepatocyte; liver; oral medicine; periodontal disease.

MeSH terms

  • Alveolar Bone Loss* / drug therapy
  • Alveolar Bone Loss* / prevention & control
  • Animals
  • Cyclohexane Monoterpenes* / pharmacology
  • Female
  • Ligation
  • Periodontitis* / drug therapy
  • Rats
  • beta-Cyclodextrins*

Substances

  • Cyclohexane Monoterpenes
  • beta-Cyclodextrins
  • alpha-terpineol