Antimicrobial susceptibility surveillance of bacterial isolates recovered in Japan from odontogenic infections in 2013

Akihiro Kaneko; Tetsuya Matsumoto; Hiroshi Iwabuchi; Junko Sato; Tomotaro Wakamura; Hiroshi Kiyota; Kazuhiro Tateda; Hideaki Hanaki; Noriyuki Sakakibara; Takayuki Mizuno; Hisashi Miyajima; Hiroyuki Naito; Ritsuo Takagi; Yasumitsu Kodama; Akira Yamaguchi; Ryo Akasiba; Nobuo Yamane; Yoshinori Jinbu; Mikio Kusama; Norito Miyagi; Reiko Kato; Noriko Nakatogawa; Kazumi Izawa; Hideki Tanzawa; Yoshinao Kozu; Hideki Watanabe; Koichi Matsumoto; Takahiko Shibahara; Yasunobu Busujima; Tsuyoshi Takato; Haruo Sakamoto; Daisuke Watanabe; Hiroyuki Kubota; Jiro Sasaki; Masataka Uematsu; Masashi Sasaki; Atsuo Kaetsu; Fumitaka Terasawa; Yoshiaki Yura; Soichi Iwai; Shosuke Morita; Kazuhiro Matsumoto; Tetsuo Oonishi; Takahide Komori; Shungo Furudoi; Junko Fujibayashi; Masahiro Urade; Hiromitsu Kishimoto; Takashi Yoshii; Takefumi Morihana; Daisuke Miyai; Tetsuji Okamoto; Taku Kanda; Kosei Okamoto; Akihiko Sakamoto; Yoshiro Matsui; Minoru Miyake; Takashi Sawai; Tetsuro Ikebe; Kenichiro Hashimoto

doi:10.1016/j.jiac.2020.05.019

Antimicrobial susceptibility surveillance of bacterial isolates recovered in Japan from odontogenic infections in 2013

J Infect Chemother. 2020 Sep;26(9):882-889. doi: 10.1016/j.jiac.2020.05.019. Epub 2020 Jun 23.

Authors

Akihiro Kaneko¹, Tetsuya Matsumoto², Hiroshi Iwabuchi³, Junko Sato², Tomotaro Wakamura², Hiroshi Kiyota², Kazuhiro Tateda², Hideaki Hanaki⁴, Noriyuki Sakakibara⁵, Takayuki Mizuno⁵, Hisashi Miyajima⁶, Hiroyuki Naito⁷, Ritsuo Takagi⁸, Yasumitsu Kodama⁸, Akira Yamaguchi⁹, Ryo Akasiba⁹, Nobuo Yamane¹⁰, Yoshinori Jinbu¹¹, Mikio Kusama¹¹, Norito Miyagi¹², Reiko Kato¹³, Noriko Nakatogawa¹⁴, Kazumi Izawa¹⁵, Hideki Tanzawa¹⁶, Yoshinao Kozu¹⁶, Hideki Watanabe¹⁷, Koichi Matsumoto¹⁷, Takahiko Shibahara¹⁸, Yasunobu Busujima¹⁹, Tsuyoshi Takato²⁰, Haruo Sakamoto²¹, Daisuke Watanabe²², Hiroyuki Kubota²², Jiro Sasaki²³, Masataka Uematsu²⁴, Masashi Sasaki²⁵, Atsuo Kaetsu²⁶, Fumitaka Terasawa²⁶, Yoshiaki Yura²⁷, Soichi Iwai²⁷, Shosuke Morita²⁸, Kazuhiro Matsumoto²⁸, Tetsuo Oonishi²⁹, Takahide Komori³⁰, Shungo Furudoi³⁰, Junko Fujibayashi³¹, Masahiro Urade³², Hiromitsu Kishimoto³², Takashi Yoshii³³, Takefumi Morihana³⁴, Daisuke Miyai³⁵, Tetsuji Okamoto³⁶, Taku Kanda³⁶, Kosei Okamoto³⁷, Akihiko Sakamoto³⁸, Yoshiro Matsui³⁹, Minoru Miyake³⁹, Takashi Sawai³⁹, Tetsuro Ikebe⁴⁰, Kenichiro Hashimoto⁴⁰

Affiliations

¹ The Surveillance Committee of Japanese Society of Chemotherapy (JSC), The Japanese Association for Infectious Diseases (JAID) and The Japanese Society for Clinical Microbiology (JSCM), Tokyo, Japan; Tokai University Hospital, Kanagawa, Japan. Electronic address: neko-1@ja2.so-net.ne.jp.
² The Surveillance Committee of Japanese Society of Chemotherapy (JSC), The Japanese Association for Infectious Diseases (JAID) and The Japanese Society for Clinical Microbiology (JSCM), Tokyo, Japan.
³ The Surveillance Committee of Japanese Society of Chemotherapy (JSC), The Japanese Association for Infectious Diseases (JAID) and The Japanese Society for Clinical Microbiology (JSCM), Tokyo, Japan; National Hospital Organization Tochigi Medical Center, Tochigi, Japan.
⁴ Infection Control Research Center, Kitasato University, Tokyo, Japan.
⁵ Department of Dentistry and Oral Surgery, Nikko Memorial Hospital, Hokkaido, Japan.
⁶ Aizu Chuo Hospital, Fukushima, Japan.
⁷ Iwaki City Medical Center, Fukushima, Japan.
⁸ Niigata University Medical & Dental Hospital, Niigata, Japan.
⁹ Nippon Dental University Niigata Hospital, Niigata, Japan.
¹⁰ Ashikaga Red Cross Hospital, Tochigi, Japan.
¹¹ Jichi Medical University Hospital, Tochigi, Japan.
¹² Nasu Red Cross Hospital, Tochigi, Japan.
¹³ Kyowa Chuo Hospital, Ibaraki, Japan.
¹⁴ Omitama City Medical Center, Ibaraki, Japan.
¹⁵ Ota Memorial Hospital, Gunma, Japan.
¹⁶ Chiba University Hospital, Chiba, Japan.
¹⁷ Yotsukaido Tokushukai Medical Center, Chiba, Japan.
¹⁸ Tokyo Dental College, Chiba, Japan.
¹⁹ Tokyo Metropolitan Bokutoh Hospital, Tokyo, Japan.
²⁰ The University of Tokyo Hospital, Tokyo, Japan.
²¹ Tokai University Hachioji Hospital, Tokyo, Japan.
²² Ikegami General Hospital, Tokyo, Japan.
²³ Jinkohkai Hospital, Kanagawa, Japan.
²⁴ Tohnoki Dental Clinic, Shizuoka, Japan.
²⁵ Shizuoka Medical Center, Shizuoka, Japan.
²⁶ Toyohashi Municipal Hospital, Aichi, Japan.
²⁷ Osaka University School of Dentistry, Osaka, Japan.
²⁸ Osaka Dental University, Osaka, Japan.
²⁹ Ikeda City Hospital, Osaka, Japan.
³⁰ Kobe University Hospital, Hyogo, Japan.
³¹ Kobe Central Hospital, Hyogo, Japan.
³² Hyogo College of Medicine Hospital, Hyogo, Japan.
³³ Yoshii Dental and Oral Surgery Clinic, Hyogo, Japan.
³⁴ Morihana Dental Clinic, Hyogo, Japan.
³⁵ Kakogawa East City Hospital, Hyogo, Japan.
³⁶ Hiroshima University Hospital, Hiroshima, Japan.
³⁷ Hiroshima City Asa Citizens Hospital, Hiroshima, Japan.
³⁸ Mazda Hospital, Hiroshima, Japan.
³⁹ Faculty of Medicine, Kagawa University, Kagawa, Japan.
⁴⁰ Fukuoka Dental College, Fukuoka, Japan.

PMID: 32591324
DOI: 10.1016/j.jiac.2020.05.019

Abstract

We report on the findings of the first antimicrobial susceptibility surveillance study in Japan of isolates recovered from odontogenic infections. Of the 38 facilities where patients representing the 4 groups of odontogenic infections were seen, 102 samples were collected from cases of periodontitis (group 1), 6 samples from pericoronitis (group 2), 84 samples from jaw inflammation (group 3) and 54 samples from phlegmon of the jaw bone area (group 4) for a total of 246 samples. The positivity rates of bacterial growth on culture were 85.3%, 100%, 84% and 88.9%, respectively, for groups 1, 2, 3 and 4. Streptococcus spp. isolation rates according to odontogenic infection group were 22% (group 1), 17.7% (group 3) and 20.7% (group 4). Anaerobic isolation rates were 66.9% (group 1), 71.8% (group 3) and 68.2% (group 4). Drug susceptibility tests were performed on 726 strains excluding 121 strains that were undergrown. The breakdown of the strains subjected to testing was 186 Streptococcus spp., 179 anaerobic gram-positive cocci, 246 Prevotella spp., 27 Porphyromonas spp., and 88 Fusobacterium spp. The isolates were tested against 30 antimicrobial agents. Sensitivities to penicillins and cephems were good except for Prevotella spp. The low sensitivities of Prevotella spp is due to β-lactamase production. Prevotella strains resistant to macrolides, quinolones, and clindamycin were found. No strains resistant to carbapenems or penems were found among all strains tested. No anaerobic bacterial strain was resistant to metronidazole. Antimicrobial susceptibility testing performed on the S. anginosus group and anaerobic bacteria, which are the major pathogens associated with odontogenic infections, showed low MIC₉₀ values to the penicillins which are the first-line antimicrobial agents for odontogenic infections; however, for Prevotella spp., penicillins combined with β-lactamase inhibitor showed low MIC₉₀ values.

Keywords: Drug Resistance; Odontogenic infection; Surveillance; Susceptibility.

MeSH terms

Anti-Bacterial Agents* / pharmacology
Anti-Bacterial Agents* / therapeutic use
Bacteria, Anaerobic
Bacterial Infections* / drug therapy
Bacterial Infections* / epidemiology
Clindamycin / pharmacology
Clindamycin / therapeutic use
Drug Resistance, Bacterial
Humans
Japan / epidemiology
Microbial Sensitivity Tests
Penicillins

Substances

Anti-Bacterial Agents
Penicillins
Clindamycin