An integration-free iPSC line ZZUNEUi008-A derived from dermal fibroblasts of a child with cardiac valvular dysplasia carrying a mutation in FLNA gene

Xiaowei Li; Yanyu Lu; Jiaxiang Wang; Mengduan Liu; Mengyu Wang; Liang Hu; Wenting Du; Lu Wang; Zhengming Jiang; Xiangmei Gu; Zhongren Ding; Deguang Feng; Ling Li; Jianzeng Dong; Xiaoyan Zhao

doi:10.1016/j.scr.2020.101882

An integration-free iPSC line ZZUNEUi008-A derived from dermal fibroblasts of a child with cardiac valvular dysplasia carrying a mutation in FLNA gene

Stem Cell Res. 2020 Jun 22:47:101882. doi: 10.1016/j.scr.2020.101882. Online ahead of print.

Authors

Xiaowei Li¹, Yanyu Lu², Jiaxiang Wang³, Mengduan Liu¹, Mengyu Wang¹, Liang Hu¹, Wenting Du¹, Lu Wang¹, Zhengming Jiang², Xiangmei Gu², Zhongren Ding¹, Deguang Feng³, Ling Li², Jianzeng Dong⁴, Xiaoyan Zhao⁵

Affiliations

¹ Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China.
² Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
³ Department of Cardiovascular Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
⁴ Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China; Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Centre for Cardiovascular Diseases, No. 2 Beijing Anzhen Road, Chaoyang District, Beijing 100029, China. Electronic address: jzdong@zzu.edu.cn.
⁵ Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China. Electronic address: fcczhaoxy8@zzu.edu.cn.

PMID: 32592953
DOI: 10.1016/j.scr.2020.101882

Abstract

FLNA gene encodes an actin-binding protein filamin A and mutations in FLNA can causes X-Linked cardiac valvular dysplasia. In this study, we report the generation of ZZUNEUi008-A, a human induced pluripotent stem cell line from a 10-year-old male patient with c. 84G → A in FLNA gene using non-integrative Sendai viral reprogramming technology. The ZZUNEUi008-A iPSC line expresses pluripotency markers, exhibits a normal male karyotype (46, XY) and can differentiate into three germ layers in vivo.