A preliminary study of dual-band confocal laser endomicroscopy combined with image mosaic in the diagnosis of liver cancer

Sheng Zheng; Ying Zhang; Shujie Chen; Zeyu Zhang; Fei Chen; Zizhen Zhang; Zhenhua Hu; Jie Tian; Liangjing Wang

doi:10.1016/j.nano.2020.102250

A preliminary study of dual-band confocal laser endomicroscopy combined with image mosaic in the diagnosis of liver cancer

Nanomedicine. 2020 Oct:29:102250. doi: 10.1016/j.nano.2020.102250. Epub 2020 Jun 30.

Authors

Sheng Zheng¹, Ying Zhang², Shujie Chen², Zeyu Zhang³, Fei Chen⁴, Zizhen Zhang⁴, Zhenhua Hu⁵, Jie Tian⁶, Liangjing Wang⁷

Affiliations

¹ Department of Gastroenterology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China; Institution of Gastroenterology, Zhejiang University, Hangzhou, China; CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.
² Institution of Gastroenterology, Zhejiang University, Hangzhou, China; Department of Gastroenterology, Sir Run Shaw Hospital, Zhejiang University, Hangzhou, China.
³ CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.
⁴ Department of Gastroenterology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China; Institution of Gastroenterology, Zhejiang University, Hangzhou, China.
⁵ CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address: zhenhua.hu@ia.ac.cn.
⁶ CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address: jie.tian@ia.ac.cn.
⁷ Department of Gastroenterology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China; Institution of Gastroenterology, Zhejiang University, Hangzhou, China. Electronic address: wangljzju@zju.edu.cn.

PMID: 32619706
DOI: 10.1016/j.nano.2020.102250

Abstract

Accurate identification of tumor tissues and their margins are still challenging for conventional clinical imaging methods during liver cancer surgery. In this study, dual-band confocal laser endomicroscopy (CLE) combined with image mosaic was used to guide liver cancer surgery. In the experiments with mice bearing orthotropic liver tumor, CLE can accurately detect the tumors and identify their margins with two excitation wavelengths of 488 nm and 660 nm by clinically available dyes fluorescein sodium (FS) or indocyanine green (ICG). The mosaic CLE images enlarged the imaging field and detected the liver tumor margins more accurately. Normal liver tissues fluorescence intensity of CLE images was significantly higher than that of tumor tissues in the same tumor-bearing mice (P < 0.0001). Overall, dual-band CLE imaging demonstrates to be a promising method to identify liver tumor tissues and margins, which has the prospect of clinical application and helps to achieve intraoperative radical resection.

Keywords: Confocal laser endomicroscopy; Fluorescein sodium; Image mosaic; Indocyanine green; Primary liver cancer; Tumor margin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line, Tumor
Heterografts
Humans
Image Processing, Computer-Assisted*
Indocyanine Green / chemistry
Liver / pathology
Liver / ultrastructure*
Liver Neoplasms / diagnostic imaging*
Liver Neoplasms / pathology
Mice
Microscopy, Confocal*

Substances

Indocyanine Green