New combination treatment from ROS-Induced sensitized radiotherapy with nanophototherapeutics to fully eradicate orthotopic breast cancer and inhibit metastasis

Te-I Liu; Ting-Yu Lu; Ying-Chieh Yang; Siou-Han Chang; Hsin-Hung Chen; I-Lin Lu; Arjun Sabu; Hsin-Cheng Chiu

doi:10.1016/j.biomaterials.2020.120229

New combination treatment from ROS-Induced sensitized radiotherapy with nanophototherapeutics to fully eradicate orthotopic breast cancer and inhibit metastasis

Biomaterials. 2020 Oct:257:120229. doi: 10.1016/j.biomaterials.2020.120229. Epub 2020 Jul 16.

Authors

Te-I Liu¹, Ting-Yu Lu¹, Ying-Chieh Yang², Siou-Han Chang¹, Hsin-Hung Chen¹, I-Lin Lu³, Arjun Sabu¹, Hsin-Cheng Chiu⁴

Affiliations

¹ Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan.
² Department of Radiology, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu, 30013, Taiwan.
³ Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan; Department of Surgery, Hsinchu Mackay Memorial Hospital, Hsinchu, 30071, Taiwan.
⁴ Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan. Electronic address: hscchiu@mx.nthu.edu.tw.

PMID: 32738654
DOI: 10.1016/j.biomaterials.2020.120229

Abstract

Radiotherapy (RT) is one of the most commonly employed approaches in the treatment of malignant tumors and is often combined with radiosensitizers to enhance the therapeutic efficacy for clinical use. For developing a smart therapeutic strategy leveraging local tissue response to photo-mediated reactions and the combination of multiple treatment modalities involving ROS-induced sensitization of RT, a novel nanophototherapeutic system has been developed. The nanotherapeutics prepared from the assembly of poly (thiodiethylene malonate) (PSDEM) and PEG-PSDEM-PEG and loaded with suberoylanilide hydroxamic acid (SAHA) employed as the RT sensitizer and indocyanine green (ICG) as the photothermal/photodynamic agent, demonstrated the capability of undergoing structural change and releasing therapeutic payloads in response to near-infrared irradiation and X-ray radiotherapy. With highly localized and controllable reactions within the tumor site, the reactive oxygen species (ROS)-triggered SAHA unloading and the hyperthermia-induced vascular permeability of oxygen led to a significant sensitization of the target tissue in RT, which, in turn, led to the promotion of therapeutic effect in conjunction with photodynamic/photothermal therapies (PDT/PTT). In vitro studies demonstrated the damage in intracellular DNA double strands and the inhibition of cell proliferation in 4T1 breast cancer cells treated with ROS-induced sensitized RT. A substantial reduction in cell viability was also observed owing to the effects of the combination of photo-mediated treatments with sensitized RT compared to the effects of RT administration alone. Complete eradication of the primary tumor and the inhibition of lung metastasis was observed in five of six orthotopic 4T1 breast cancer-bearing mice subjected to combined PDT/PTT in nanophototherapeutics with ROS-induced sensitized RT at a low dosage (6 Gy), leading to the prominent survival fraction of ca. 83% over 60 days.

Keywords: Combination therapy; Photothermal/photodynamic therapies; Radiosensitizer; Radiotherapy; Stimulus-induced drug release.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Breast Neoplasms* / drug therapy
Cell Line, Tumor
Humans
Hyperthermia, Induced*
Indocyanine Green
Mice
Photochemotherapy*
Phototherapy
Reactive Oxygen Species

Substances

Reactive Oxygen Species
Indocyanine Green