Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study

Sara Darbandi; Mahsa Darbandi; Ashok Agarwal; Hamid Reza Khorram Khorshid; Mohammad Reza Sadeghi; Sandro C Esteves; Pallav Sengupta; Sulagna Dutta; Zohreh Fathi; Hojjat Zeraati; Mohammad Mehdi Akhondi

doi:10.18502/ijrm.v13i6.7284

Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study

Int J Reprod Biomed. 2020 Jun 30;18(6):425-438. doi: 10.18502/ijrm.v13i6.7284. eCollection 2020 Jun.

Affiliations

¹ Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
² American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio, USA.
³ Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
⁴ Androfert, Andrology and Human Reproduction Clinic, Campinas, Brazil.
⁵ Department of Physiology, Faculty of Medicine, Mahsa University, Selangor, Malaysia.
⁶ Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Background: The three-parent assisted reproductive technique may increase oocyte competence.

Objective: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asynchronous ooplasmic transfer using waste MII oocyte (waOT) for maturation of the human-aged non-surrounded nucleolus germinal vesicle-stage (NSN-GV) oocyte were investigated.

Materials and methods: NSN-GV oocytes were subjected to four methods: group A (GVT), B (sOT), C (caOT) D (waOT), and E (Control). The fusion rates, MI, MII, ICSI observations and cleavage at 2-cell, 4-cell, and 8-cell stages were compared in the groups.

Results: In GVT, none of the oocytes fused. In sOT, all oocytes fused, 20 achieved the MI, 14 progressed to MII, 8 fertilized, 6 cleaved and 5, 4, and 3 achieved the 2-cells, 4-cells and 8-cells, respectively. In caOT, all oocytes fused and achieved the MI, 8 progressed to MII and fertilized, 6 cleaved and 6, 5, and 5 achieved the 2-cells, 4-cells, and 8-cells respectively. In waOT, all oocytes fused, 5 and 3 progressed to MI and MII, respectively, but only one fertilized, cleaved and reached a 4-cells stage. In group E, 6 and 2 oocytes progressed to MI and MII, respectively, and only one fertilized but arrested at the zygote stage. caOT had the highest survival rate when compared to sOT (p = 0.04), waOT (p = 0.002), and control (p = 0.001).

Conclusion: The caOT method was beneficial over sOT, waOT, and GVT in supplementing the developmental capacity of human-aged NSN-GV oocytes.

Keywords: In vitro oocyte maturation techniques; Nuclear transfer techniques; Oocyte donation.; Oocytes; Assisted reproductive techniques.