Generation and Culture of Peripheral CD34+ Stem Cell-Derived Mast Cells (PSCMCs)

Yanyan Luo; Stefan Frischbutter; Jörg Scheffel; Frank Siebenhaar

doi:10.1007/978-1-0716-0696-4_5

Generation and Culture of Peripheral CD34⁺ Stem Cell-Derived Mast Cells (PSCMCs)

Methods Mol Biol. 2020:2163:63-67. doi: 10.1007/978-1-0716-0696-4_5.

Authors

Yanyan Luo¹, Stefan Frischbutter¹, Jörg Scheffel¹, Frank Siebenhaar²

Affiliations

¹ Department of Dermatology and Allergy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
² Department of Dermatology and Allergy, Charité-Universitätsmedizin Berlin, Berlin, Germany. frank.siebenhaar@charite.de.

PMID: 32766966
DOI: 10.1007/978-1-0716-0696-4_5

Abstract

Mast cells (MCs) are long-living tissue-resident cells that play an important role in inflammatory and allergic reactions. In vitro models of mast cell functions have allowed better understanding of the function of mast cells and mast cell-mediated disorders. In this unit, we describe a protocol used for the generation and culture of peripheral CD34⁺ stem cell-derived mast cells (PSCMCs). It provides a useful tool for the investigation of the biology of human MCs in vitro.

Keywords: CD117; CD34; Stem cell-derived mast cells.

MeSH terms

Antigens, CD34 / genetics
Antigens, CD34 / metabolism
Cell Differentiation
Cells, Cultured
Humans
Mast Cells / cytology*
Mast Cells / metabolism
Peripheral Blood Stem Cells / cytology*
Peripheral Blood Stem Cells / metabolism
Primary Cell Culture / methods*

Substances

Antigens, CD34