A "Western Diet" promotes symptoms of hepatic steatosis in spontaneously hypertensive rats

Int J Exp Pathol. 2020 Oct;101(5):152-161. doi: 10.1111/iep.12369. Epub 2020 Aug 12.

Abstract

Systemic hypertension, characterized by elevated blood pressure ≥140/90 mm Hg, is a major modifiable risk factor for cardiovascular disease. Hypertension also associates with non-alcoholic fatty liver disease (NAFLD), which is becoming common due to a modern diet and lifestyle. The aim of the present study was to examine whether a high-fat "Western" diet had effects on hypertension and associated NAFLD. Normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR) were placed on a normal chow or high-fat diet for 8 weeks; blood pressure was measured fortnightly and body weight recorded weekly. As expected, SHR had elevated blood pressure compared to WKY. Diet did not influence blood pressure. Compared to SHR, WKY rats gained more weight, associating with increased white adipose tissue weight. Normotensive rats also had higher plasma cholesterol and triglycerides in response to a "Western" diet, with no changes in plasma glucose levels. Neither strain developed atherosclerosis. Interestingly, high-fat diet-fed SHR had increased liver weight, associating with a significant level of hepatic lipid accumulation not observed in WKY. Further, they exhibited hepatocellular ballooning and increased hepatic inflammation, indicative of steatohepatitis. These findings suggest that a high-fat "Western" diet promotes features of NAFLD in SHR, but not WKY rats. Importantly, the high-fat diet had no effect on blood pressure.

Keywords: hepatic steatosis; hypertension; liver.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Pressure
  • Cholesterol / blood
  • Diet, High-Fat / adverse effects*
  • Fatty Liver / etiology*
  • Fatty Liver / physiopathology
  • Hypertension / complications*
  • Hypertension / physiopathology
  • Liver / physiopathology
  • Male
  • Non-alcoholic Fatty Liver Disease / complications*
  • Non-alcoholic Fatty Liver Disease / etiology*
  • Rats
  • Rats, Inbred SHR
  • Rats, Inbred WKY
  • Triglycerides / blood

Substances

  • Triglycerides
  • Cholesterol