HPC-REDItools: a novel HPC-aware tool for improved large scale RNA-editing analysis

BMC Bioinformatics. 2020 Aug 21;21(Suppl 10):353. doi: 10.1186/s12859-020-03562-x.

Abstract

Background: RNA editing is a widespread co-/post-transcriptional mechanism that alters primary RNA sequences through the modification of specific nucleotides and it can increase both the transcriptome and proteome diversity. The automatic detection of RNA-editing from RNA-seq data is computational intensive and limited to small data sets, thus preventing a reliable genome-wide characterisation of such process.

Results: In this work we introduce HPC-REDItools, an upgraded tool for accurate RNA-editing events discovery from large dataset repositories.

Availability: https://github.com/BioinfoUNIBA/REDItools2 .

Conclusions: HPC-REDItools is dramatically faster than the previous version, REDItools, enabling big-data analysis by means of a MPI-based implementation and scaling almost linearly with the number of available cores.

Keywords: Bioinformatics pipeline; High Performance Computing; Next Generation Sequencing; REDItools; RNA-editing; Sequence Analysis.

MeSH terms

  • Algorithms
  • Base Sequence
  • Computing Methodologies*
  • Genome
  • RNA Editing / genetics*
  • Software*
  • Transcriptome / genetics