Comparative assessment of faecal diagnostics for detection and predictive modelling of endemic Fasciola hepatica infection in sheep and cattle on Australian farms

S D George; A J George; P F Rolfe; D L Emery

doi:10.1016/j.vpoa.2018.100001

Comparative assessment of faecal diagnostics for detection and predictive modelling of endemic Fasciola hepatica infection in sheep and cattle on Australian farms

Vet Parasitol X. 2019 Jan 16:1:100001. doi: 10.1016/j.vpoa.2018.100001. eCollection 2019 May.

Authors

S D George^{1

2}, A J George², P F Rolfe², D L Emery¹

Affiliations

¹ Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, New South Wales, Australia.
² Elanco Animal Health, Yarrandoo R&D Centre, 245 Western Road, Kemps Creek, NSW, 2178, Australia.

Abstract

The diagnosis, monitoring and flukicide efficacy testing of fasciolosis on-farm is reliant on non-terminal methods. The coproantigen ELISA (cELISA) has been recommended for diagnosis of fasciolosis and associated flukicide efficacy testing as an alternative to fluke egg counts for monitoring parasitism. Recently experimental multi-age infections have suggested that the reliability of efficacy results can be improved by a second cELISA testing at 6 weeks post-treatment (wpt) in addition to the generally accepted 1 wpt. A field study was conducted to determine the suitability of faecal fluke egg counts (FFEC) and cELISA as diagnostic, drug efficacy testing and epidemiological tools on Australian sheep and cattle farms. Faecal samples from sheep and/or cattle on three endemic farms were taken at monthly intervals for 12 months and examined by both methods. Normal farm management was maintained during the study period and opportunistic efficacy testing, in line with each farm's normal flukicide management was undertaken. Additionally, the suitability of the Ollerenshaw Index as a predictive model for fasciolosis under Australian conditions was examined. While both diagnostics demonstrated their value in the farm environment, the current data demonstrate a distinct and significant increase in diagnostic sensitivity for epidemiological studies by using the two tests in parallel. The agreement between the two diagnostics was found to be higher in cattle, despite the poor sensitivity of FFEC in this species. Similar levels of agreement between the two tests were demonstrated at both sheep properties, regardless of the marked difference in the intensity of F. hepatica challenge. Linear regression models demonstrated the results of the two diagnostics utilized in parallel were explained substantially (R² = 0.91) as were series data (R² = 0.88) when the respective models were fitted. In contrast, the fitted models for FFEC (R² = 0.54) and cELISA (R² = 0.58) were poor explanations for test outcomes. The outcomes of these models support previous findings that suggest that the two diagnostic tests are best utilized together, particularly in parallel. The application of the Ollerenshaw Index to Australian conditions requires further investigation.

Keywords: Coproantigen; ELISA; Efficacy; Fasciola hepatica; Prevalence; Triclabendazole.