Mitochondrial Mechanisms of LRRK2 G2019S Penetrance

Sylvie Delcambre; Jenny Ghelfi; Nassima Ouzren; Léa Grandmougin; Catherine Delbrouck; Philip Seibler; Kobi Wasner; Jan O Aasly; Christine Klein; Joanne Trinh; Sandro L Pereira; Anne Grünewald

doi:10.3389/fneur.2020.00881

Mitochondrial Mechanisms of LRRK2 G2019S Penetrance

Front Neurol. 2020 Aug 25:11:881. doi: 10.3389/fneur.2020.00881. eCollection 2020.

Authors

Affiliations

¹ Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.
² Department of Oncology, Luxembourg Institute of Health, Luxembourg, Luxembourg.
³ Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.
⁴ Department of Neuromedicine and Movement Science, Department of Neurology, St. Olav's Hospital, Norwegian University of Science and Technology, Trondheim, Norway.

Abstract

Several mutations in leucine-rich repeat kinase-2 (LRRK2) have been associated with Parkinson's disease (PD). The most common substitution, G2019S, interferes with LRRK2 kinase activity, which is regulated by autophosphorylation. Yet, the penetrance of this gain-of-function mutation is incomplete, and thus far, few factors have been correlated with disease status in carriers. This includes (i) LRRK2 autophosphorylation in urinary exosomes, (ii) serum levels of the antioxidant urate, and (iii) abundance of mitochondrial DNA (mtDNA) transcription-associated 7S DNA. In light of a mechanistic link between LRRK2 kinase activity and mtDNA lesion formation, we previously investigated mtDNA integrity in fibroblasts from manifesting (LRRK2+/PD+) and non-manifesting carriers (LRRK2+/PD-) of the G2019S mutation as well as from aged-matched controls. In our published study, mtDNA major arc deletions correlated with PD status, with manifesting carriers presenting the highest levels. In keeping with these findings, we now further explored mitochondrial features in fibroblasts derived from LRRK2+/PD+ (n = 10), LRRK2+/PD- (n = 21), and control (n = 10) individuals. In agreement with an accumulation of mtDNA major arc deletions, we also detected reduced NADH dehydrogenase activity in the LRRK2+/PD+ group. Moreover, in affected G2019S carriers, we observed elevated mitochondrial mass and mtDNA copy numbers as well as increased expression of the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2), which regulates antioxidant signaling. Taken together, these results implicate mtDNA dyshomeostasis-possibly as a consequence of impaired mitophagy-in the penetrance of LRRK2-associated PD. Our findings are a step forward in the pursuit of unveiling markers that will allow monitoring of disease progression of LRRK2 mutation carriers.

Keywords: G2019S; Parkinson's disease; fibroblasts; leucine-rich repeat kinase-2 (LRRK2); mitochondria; mitochondrial DNA (mtDNA); penetrance.