Does resveratrol affect prepared sperm parameters and chromatin quality in normozoospermic and asthenozoospermic patients before and after freezing? A lab trial study

Masoomeh Mohammadzadeh; Mohammad Ali Khalili; Vahid Ramezani; Hamed Hamishehkar; Laleh Dehghan Marvast; Esmat Mangoli; Mahya Rajabi; Zhima Akhavan Sales; Ali Reza Talebi

doi:10.18502/ijrm.v13i9.7670

Does resveratrol affect prepared sperm parameters and chromatin quality in normozoospermic and asthenozoospermic patients before and after freezing? A lab trial study

Int J Reprod Biomed. 2020 Sep 20;18(9):755-764. doi: 10.18502/ijrm.v13i9.7670. eCollection 2020 Sep.

Authors

Masoomeh Mohammadzadeh¹, Mohammad Ali Khalili¹, Vahid Ramezani², Hamed Hamishehkar³, Laleh Dehghan Marvast¹, Esmat Mangoli¹, Mahya Rajabi⁴, Zhima Akhavan Sales⁵, Ali Reza Talebi¹

Affiliations

¹ Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
² Department of Pharmaceutics, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
³ Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁴ Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
⁵ Department of Immunology, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Abstract

Background: Previous studies have examined the effect of resveratrol as a potent antioxidant for free radicals in semen. While, the prepared spermatozoa are more affected by ROS factors due to centrifugation and incubation.

Objective: To evaluate the RSV's effects on the prepared sperm parameters and chromatin quality in both normozoospermic and asthenozoospermic cases before and after freezing.

Materials and methods: The sample of 10 normozoospermic and asthenozoospermic men was prepared through the swim-up method. The groups were then divided into two samples of control and experimental (exposure to 30 µmol/l of RSV) to evaluate and compare the sperm parameters and chromatin quality before and after freezing.

Results: The motility and viability of spermatozoa were seen to be significantly different before and after freezing separately in the control and treatment samples of the groups (p $\leq$ 0.001 and p = 0.001, respectively). However, the stated difference between the control and treatment samples of normozoospermic and asthenozoospermic patients were not significant (p $>$ 0.05). In addition, the sperm morphology and chromatin quality were not significantly different between the two samples of each group; nonetheless, chromatin quality of the treated sample was better than that of the control before and after freezing.

Conclusion: Despite the protective effects of RSV on the semen samples, RSV cannot affect significantly the prepared sperms parameters and chromatin quality in normozoospermic and asthenozoospermic patients.

Keywords: Chromatin; Freeze; Motility; Resveratrol; Spermatozoa.