Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells

Roza Maria Barouni; Chiara Musiu; Vincenzo Bronte; Stefano Ugel; Stefania Canè

doi:10.1007/978-1-0716-1060-2_4

Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells

Methods Mol Biol. 2021:2236:29-42. doi: 10.1007/978-1-0716-1060-2_4.

Authors

Roza Maria Barouni¹, Chiara Musiu¹, Vincenzo Bronte², Stefano Ugel¹, Stefania Canè¹

Affiliations

¹ Department of Medicine, Section of Immunology, University of Verona, Verona, Italy.
² Department of Medicine, Section of Immunology, University of Verona, Verona, Italy. vincenzo.bronte@univr.it.

PMID: 33237538
DOI: 10.1007/978-1-0716-1060-2_4

Abstract

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous cell population composed of mature and immature cells of myeloid origin that play a major role in tumor progression by inhibiting the antitumor immune responses mediated by T cells. In this chapter, we describe protocols for isolation, phenotypical and functional evaluation of MDSCs isolated from mouse tumors, with the aim at unifying and standardizing protocols set up by different laboratories.

Keywords: Cell sorting; ELISPOT; Immunosuppression; Myeloid-derived suppressor cells (MDSCs); Tumor digestion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Separation / methods*
Flow Cytometry
Immunophenotyping / methods*
Interferon-gamma / metabolism
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Myeloid-Derived Suppressor Cells / pathology*
Neoplasms / pathology*
Phenotype
Reactive Oxygen Species / metabolism
T-Lymphocytes / metabolism

Substances

Reactive Oxygen Species
Interferon-gamma