Study of haemolysis interference limit on serum potassium assay on Roche® Cobas 8000 and evaluation of corrected potassium

Scand J Clin Lab Invest. 2021 Feb;81(1):82-84. doi: 10.1080/00365513.2020.1855363. Epub 2020 Dec 3.

Abstract

Roche Diagnostics® decided a more restrictive haemolysis index (≤20 HI) for approval the release of serum potassium results. This study examined the risk of overestimating serum potassium results related to the HI according to the Ricos total error (±5.6%) and evaluated the approach of Martinez-Morillo and Alvarez to determine a corrected potassium. According to Ricos' criteria, our study showed compliant potassium results with HI less than or equal to 75. Between 90 and 100 HI, the results did not show a significant overestimation. The equation to obtain Kcorrected was: Kmeasured - (0.004 × HI). The use of corrective formulas for adjusting results of potassium could help the laboratory to identify patients at increased risk and to repeat the test as soon as possible.

Keywords: Potassium; analytical interference; correcting results; haemolysis index; overestimation.

MeSH terms

  • Confidence Intervals
  • Hematologic Tests / instrumentation*
  • Hemolysis*
  • Linear Models
  • Potassium / blood*

Substances

  • Potassium