Bioinspired membrane provides periosteum-mimetic microenvironment for accelerating vascularized bone regeneration

Gaojie Yang; Haoming Liu; Yi Cui; Jiaqi Li; Xuan Zhou; Nuoxin Wang; Feige Wu; Yan Li; Yu Liu; Xingyu Jiang; Shengmin Zhang

doi:10.1016/j.biomaterials.2020.120561

Bioinspired membrane provides periosteum-mimetic microenvironment for accelerating vascularized bone regeneration

Biomaterials. 2021 Jan:268:120561. doi: 10.1016/j.biomaterials.2020.120561. Epub 2020 Dec 1.

Authors

Gaojie Yang¹, Haoming Liu², Yi Cui³, Jiaqi Li⁴, Xuan Zhou⁴, Nuoxin Wang⁵, Feige Wu⁴, Yan Li⁴, Yu Liu⁴, Xingyu Jiang⁶, Shengmin Zhang⁷

Affiliations

¹ Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China; Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China; Media Lab and McGovern Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
² Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China; Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China; Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
³ Media Lab and McGovern Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
⁴ Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China; Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
⁵ Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.
⁶ Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China. Electronic address: xingyujiang@nanoctr.cn.
⁷ Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China; Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China. Electronic address: smzhang@hust.edu.cn.

PMID: 33316630
DOI: 10.1016/j.biomaterials.2020.120561

Abstract

Periosteum plays a pivotal role in vascularization, ossification and remodeling during the healing process of bone injury. However, there are few studies focused on the construction of artificial implants with periosteum-mimetic effect. To emulate the primary role of natural periosteum or endosteal tissues in bone regeneration, here we provide a functional biomimetic membrane with micropatterns of site-speciﬁc biomineralization. The micropattern is generated by using printed hydroxyapatite nanoparticles (HANPs), combined with selective growth of biomineralized apatite and in situ coprecipitation with growth factors. The biomimetic membrane can sustainably provide a periosteum-mimetic microenvironment, such as long-term topographical guidance for cell recruitment and induced cell differentiation, by releasing calcium phosphate and growth factors. We demonstrated that rat mesenchymal stem cells (rMSCs) on such biomimetic membrane exhibited highly aligned organization, leading to enhanced angiogenesis and osteogenesis. In the rat calvarial defect model, our biomimetic membranes with biomineralized micropatterns could significantly enhance vascularized ossification and accelerate new bone formation. The current work suggests that the functionally biomimetic membranes with specific biomineralized micropatterns can be a promising alternative to periosteal autografts, with great potential for bench-to-bedside translation in orthopedics.

Keywords: Biomimetic periosteum; Bone regeneration; Cell manipulation; Hydroxyapatite; Micropattern.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biomimetics*
Bone Regeneration
Osteogenesis
Periosteum*
Rats
Tissue Engineering