Colorimetric detection and bio-magnification of bisphenol A in fish organs and water sources using 3',6'-bis(diethylamino)-2- ((3,4,5trimethyl benzylidene) amino) spiro [isoindoline -1,9'-xanthen ]-3-one (BTSIXO)-Fe3+ ion conjugate

Abstract

Current study is focusing mainly on the development of simple, novel, and cost-effective optical sensor to detect and quantify Bisphenol A (BPA) contamination. We designed a very selective and sensitive colorimetric sensor using synthesized 3', 6'- bis(diethylamino) -2- ((3,4,5 trimethyl benzylidene) amino)spiro [isoindoline-1,9'-xanthen] -3-one (BTSIXO) conjugated with Fe³⁺-ions via very simple eco- friendly synthetic protocol. The sensor has an excellent wide detection range for BPA from 0.1 to 150 ppm with LODs of 0.02 ppm. Finally, the applicability of the sensor was demonstrated in fish samples especially in the organs of Oreochromis mossambicus fingerlings and contaminated industrial water samples. The sensor was also applied for the quantification of BPA present drinking water stored in the plastic bottles. The developed sensor has shown a good agreement and accuracy when compared with ESI-Mass techniques.

Keywords: BTSIXO; Bisphenol A; Colorimetric; Conjugate; Estrogen; Histopathology; One pot synthesis; Oreochromismossambicus.