Chronic Ethanol Exposure Potentiates Cholinergic Neurotransmission in the Basolateral Amygdala

Neuroscience. 2021 Feb 10:455:165-176. doi: 10.1016/j.neuroscience.2020.12.014. Epub 2020 Dec 29.

Abstract

Chronic intermittent ethanol (CIE) exposure dysregulates glutamatergic and GABAergic neurotransmission, facilitating basolateral amygdala (BLA) pyramidal neuron hyperexcitability and the expression of anxiety during withdrawal. It is unknown whether ethanol-induced alterations in nucleus basalis magnocellularis (NBM) cholinergic projections to the BLA mediate anxiety-related behaviors through direct modulation of GABA and glutamate afferents. Following 10 days of CIE exposure and 24 h of withdrawal, we recorded GABAergic and glutamatergic synaptic responses in BLA pyramidal neurons with electrophysiology, assessed total protein expression of cholinergic markers, and quantified acetylcholine and choline concentrations using a colorimetric assay. We measured α7 nicotinic acetylcholine receptor (nAChR) dependent modulation of presynaptic function at distinct inputs in AIR- and CIE-exposed BLA coronal slices as a functional read-out of cholinergic neurotransmission. CIE/withdrawal upregulates the endogenous activity of α7 nAChRs, facilitating release at both GABAergic' local' interneuron and glutamatergic synaptic responses to stria terminalis (ST) stimulation, with no effect at GABAergic lateral paracapsular cells (LPCs). CIE caused a three-fold increase in BLA acetylcholine concentration, with no changes in α7 nAChR or cholinergic marker expression. These data illustrate that α7 nAChR-dependent changes in presynaptic function serve as a proxy for CIE-dependent alterations in synaptic acetylcholine levels. Thus, cholinergic projections appear to mediate CIE-induced alterations at GABA/glutamate inputs.

Keywords: acetylcholine; basolateral amygdala; patch-clamp electrophysiology; presynaptic; withdrawal.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amygdala* / drug effects
  • Amygdala* / physiopathology
  • Animals
  • Basolateral Nuclear Complex*
  • Cholinergic Agents / pharmacology
  • Ethanol* / pharmacology
  • Excitatory Postsynaptic Potentials
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Synapses
  • Synaptic Transmission

Substances

  • Cholinergic Agents
  • Ethanol