Structural determination of molecular complexes by cryo-EM requires large, often complex processing of the image data that are initially obtained. Here, TEMPy2, an update of the TEMPy package to process, optimize and assess cryo-EM maps and the structures fitted to them, is described. New optimization routines, comprehensive automated checks and workflows to perform these tasks are described.
Keywords: TEMPy2; fitting scores; macromolecular complexes; model assessment; model fitting; three-dimensional electron microscopy; validation.
open access.