Expanding the Nude SCID/CID Phenotype Associated with FOXN1 Homozygous, Compound Heterozygous, or Heterozygous Mutations

J Clin Immunol. 2021 May;41(4):756-768. doi: 10.1007/s10875-021-00967-y. Epub 2021 Jan 19.

Abstract

Human nude SCID is a rare autosomal recessive inborn error of immunity (IEI) characterized by congenital athymia, alopecia, and nail dystrophy. Few cases have been reported to date. However, the recent introduction of newborn screening for IEIs and high-throughput sequencing has led to the identification of novel and atypical cases. Moreover, immunological alterations have been recently described in patients carrying heterozygous mutations. The aim of this paper is to describe the extended phenotype associated with FOXN1 homozygous, compound heterozygous, or heterozygous mutations. We collected clinical and laboratory information of a cohort of 11 homozygous, 2 compound heterozygous, and 5 heterozygous patients with recurrent severe infections. All, except one heterozygous patient, had signs of CID or SCID. Nail dystrophy and alopecia, that represent the hallmarks of the syndrome, were not always present, while almost 50% of the patients developed Omenn syndrome. One patient with hypomorphic compound heterozygous mutations had a late-onset atypical phenotype. A SCID-like phenotype was observed in 4 heterozygous patients coming from the same family. A spectrum of clinical manifestations may be associated with different mutations. The severity of the clinical phenotype likely depends on the amount of residual activity of the gene product, as previously observed for other SCID-related genes. The severity of the manifestations in this heterozygous family may suggest a mechanism of negative dominance of the specific mutation or the presence of additional mutations in noncoding regions.

Keywords: EBV-related lymphoproliferative disease; FOXN1; Nude SCID; Omenn syndrome; alopecia; compound heterozygous; heterozygous; homozygous; nail dystrophy.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Child, Preschool
  • DNA Mutational Analysis
  • Disease Management
  • Female
  • Forkhead Transcription Factors / chemistry
  • Forkhead Transcription Factors / genetics*
  • Genetic Association Studies
  • Genetic Loci
  • Genetic Predisposition to Disease
  • Hematopoietic Stem Cell Transplantation
  • Heterozygote*
  • High-Throughput Nucleotide Sequencing
  • Homozygote*
  • Humans
  • Male
  • Models, Molecular
  • Molecular Conformation
  • Mutation*
  • Pedigree
  • Phenotype*
  • Severe Combined Immunodeficiency / diagnosis*
  • Severe Combined Immunodeficiency / etiology*
  • Severe Combined Immunodeficiency / therapy
  • Structure-Activity Relationship
  • Treatment Outcome

Substances

  • Forkhead Transcription Factors
  • Whn protein