The influence of agar brands and micronutrients in the growth optimization of Granulicella sp. (Acidobacteriota)

Ohana Y A Costa; Eiko E Kuramae

doi:10.1016/j.mimet.2021.106148

The influence of agar brands and micronutrients in the growth optimization of Granulicella sp. (Acidobacteriota)

J Microbiol Methods. 2021 Feb:181:106148. doi: 10.1016/j.mimet.2021.106148. Epub 2021 Jan 20.

Authors

Ohana Y A Costa¹, Eiko E Kuramae²

Affiliations

¹ Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, Droevendaalsesteeg 10, 6708, PB, Wageningen, the Netherlands. Electronic address: o.costa@nioo.knaw.nl.
² Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, Droevendaalsesteeg 10, 6708, PB, Wageningen, the Netherlands; Institute of Environmental Biology, Ecology and Biodiversity, Utrecht University, 3584, CH, Utrecht, the Netherlands.

PMID: 33484740
DOI: 10.1016/j.mimet.2021.106148

Abstract

Acidobacteriota are highly abundant in soils, however, few cultured representatives are available. The purity of the reagents can influence microbial growth in laboratory conditions and successful isolation. Here we investigated the impact of different agar brands in culture medium and advocate that agar origin should be carefully considered for Acidobacteriota strains growth and microbial isolation.

Keywords: Acidobacteriota; Agar brand; Metals; Microbial growth; Micronutrients.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acidobacteria / growth & development*
Agar / metabolism
Culture Media* / chemistry
Culture Media* / metabolism
Micronutrients / metabolism*

Substances

Culture Media
Micronutrients
Agar