Improving the Cpf1-mediated base editing system by combining dCas9/dead sgRNA with human APOBEC3A variants

Meng Lian; Fangbing Chen; Xingyun Huang; Xiaozhu Zhao; Shixue Gou; Nan Li; Qin Jin; Hui Shi; Yanhui Liang; Jingke Xie; Weikai Ge; Zhenpeng Zhuang; Jiaowei Wang; Yinghua Ye; Yi Yang; Kepin Wang; Liangxue Lai; Han Wu

doi:10.1016/j.jgg.2020.07.010

Improving the Cpf1-mediated base editing system by combining dCas9/dead sgRNA with human APOBEC3A variants

J Genet Genomics. 2021 Jan 20;48(1):92-95. doi: 10.1016/j.jgg.2020.07.010. Epub 2020 Nov 7.

Authors

Meng Lian¹, Fangbing Chen², Xingyun Huang², Xiaozhu Zhao², Shixue Gou², Nan Li², Qin Jin², Hui Shi², Yanhui Liang², Jingke Xie², Weikai Ge², Zhenpeng Zhuang², Jiaowei Wang², Yinghua Ye³, Yi Yang⁴, Kepin Wang⁵, Liangxue Lai⁶, Han Wu⁷

Affiliations

¹ CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, China.
² CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; University of Chinese Academy of Sciences, Beijing 100049, China.
³ CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou 510530, China; Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou 510005, China.
⁴ Key Laboratory for Major Obstetrics Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Hospital Affiliated of Guangzhou Medical University, Guangzhou 510150, China.
⁵ CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou 510530, China; Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou 510005, China. Electronic address: wang_kepin@gibh.ac.cn.
⁶ CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou 510530, China; Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou 510005, China. Electronic address: lai_liangxue@gibh.ac.cn.
⁷ CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019RU015), Guangzhou 510530, China; Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou 510005, China. Electronic address: wu_han@gibh.ac.cn.

PMID: 33504469
DOI: 10.1016/j.jgg.2020.07.010

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Associated Protein 9* / genetics
CRISPR-Cas Systems / genetics
Cytidine Deaminase* / genetics
Gene Editing
Humans
Proteins

Substances

Proteins
CRISPR-Associated Protein 9
APOBEC3A protein, human
Cytidine Deaminase