Antibody response in patients admitted to the hospital with suspected SARS-CoV-2 infection: results from a multicenter study across Spain

Eur J Clin Microbiol Infect Dis. 2021 Jun;40(6):1343-1349. doi: 10.1007/s10096-020-04139-5. Epub 2021 Jan 29.

Abstract

Aim: To evaluate the serological response against SARS-CoV-2 in a multicenter study representative of the Spanish COVID pandemic.

Methods: IgG and IgM + IgA responses were measured on 1466 samples from 1236 Spanish COVID-19 patients admitted to the hospital, two commercial ELISA kits (Vircell SL, Spain) based on the detection of antibodies against the viral spike protein and nucleoprotein, were used.

Results: Approximately half of the patients presented antibodies (56.8% were IgM + IgA positive and 43.0% were IgG positive) as soon as 2 days after the first positive PCR result. Serological test positivity increased with time from the PCR test, and 10 days after the first PCR result, 91.5% and 88.0% of the patients presented IgM + IgA and IgG antibodies, respectively.

Conclusion: The high values of sensitivity attained in the present study from a relatively early period of time after hospitalization support the use of the evaluated serological assays as supplementary diagnostic tests for the clinical management of COVID-19.

Keywords: COVID-19; Diagnosis; IgA; IgG; IgM; SARS-COV-2.

Publication types

  • Multicenter Study

MeSH terms

  • Adolescent
  • Adult
  • Age Factors
  • Aged
  • Aged, 80 and over
  • Antibodies, Viral / blood*
  • Antibody Formation*
  • COVID-19 / immunology*
  • COVID-19 Serological Testing
  • Coronavirus Nucleocapsid Proteins / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Hospitalization
  • Humans
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Male
  • Middle Aged
  • Phosphoproteins / immunology
  • Sensitivity and Specificity
  • Sex Factors
  • Spain
  • Spike Glycoprotein, Coronavirus / immunology
  • Young Adult

Substances

  • Antibodies, Viral
  • Coronavirus Nucleocapsid Proteins
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Phosphoproteins
  • Spike Glycoprotein, Coronavirus
  • nucleocapsid phosphoprotein, SARS-CoV-2
  • spike protein, SARS-CoV-2