Identification of a Novel Hybrid Plasmid Encoding KPC-2 and Virulence Factors in Klebsiella pneumoniae Sequence Type 11

Longyang Jin; Ruobing Wang; Hua Gao; Qi Wang; Hui Wang

doi:10.1128/AAC.02435-20

Identification of a Novel Hybrid Plasmid Encoding KPC-2 and Virulence Factors in Klebsiella pneumoniae Sequence Type 11

Antimicrob Agents Chemother. 2021 May 18;65(6):e02435-20. doi: 10.1128/AAC.02435-20. Print 2021 May 18.

Authors

Longyang Jin¹, Ruobing Wang¹, Hua Gao¹, Qi Wang¹, Hui Wang²

Affiliations

¹ Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.
² Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China whuibj@163.com.

Abstract

Recent emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) coharboring bla_KPC-2 and pLVPK-like virulence plasmids represented a novel clinical challenge. In the present study, we characterized a bla_KPC-2 and virulence hybrid plasmid, designated pCRHV-C2244, from a clinical ST11-K64 CRKP strain. pCRHV-C2244 was non-self-transmissible due to incomplete conjugative elements but mobilizable together with a conjugative helper. Enhanced virulence and stable maintenance without significant fitness loss in its original host were confirmed in vitro and in vivo.

Keywords: CRKP; IS26; fitness cost; hybrid plasmid; virulence determinants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Klebsiella Infections*
Klebsiella pneumoniae* / genetics
Plasmids / genetics
Virulence Factors / genetics
beta-Lactamases / genetics

Substances

Virulence Factors
beta-Lactamases