Molecular characterization of high-risk human papillomavirus (HR-HPV) in women in Lomé, Togo

BMC Infect Dis. 2021 Mar 19;21(1):278. doi: 10.1186/s12879-021-05956-5.

Abstract

Background: The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women.

Methods: Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR.

Results: Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL.

Conclusion: This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.

Keywords: Genotypes; HR-HPV; Lomé; PCR; Togo.

MeSH terms

  • Adult
  • Alphapapillomavirus / genetics*
  • Alphapapillomavirus / isolation & purification
  • Cervix Uteri / virology
  • Cross-Sectional Studies
  • DNA, Viral / genetics
  • DNA, Viral / metabolism
  • Female
  • Genotype
  • Humans
  • Middle Aged
  • Multiplex Polymerase Chain Reaction
  • Papillomavirus Infections / epidemiology
  • Papillomavirus Infections / pathology*
  • Risk Factors
  • Togo / epidemiology

Substances

  • DNA, Viral