MicroRNA-30 regulates left ventricular hypertrophy in chronic kidney disease

JCI Insight. 2021 May 24;6(10):e138027. doi: 10.1172/jci.insight.138027.

Abstract

Left ventricular hypertrophy (LVH) is a primary feature of cardiovascular complications in patients with chronic kidney disease (CKD). miRNA-30 is an important posttranscriptional regulator of LVH, but it is unknown whether miRNA-30 participates in the process of CKD-induced LVH. In the present study, we found that CKD not only resulted in LVH but also suppressed miRNA-30 expression in the myocardium. Rescue of cardiomyocyte-specific miRNA-30 attenuated LVH in CKD rats without altering CKD progression. Importantly, in vivo and in vitro knockdown of miRNA-30 in cardiomyocytes led to cardiomyocyte hypertrophy by upregulating the calcineurin signaling directly. Furthermore, CKD-related detrimental factors, such as fibroblast growth factor-23, uremic toxin, angiotensin II, and transforming growth factor-β, suppressed cardiac miRNA-30 expression, while miRNA-30 supplementation blunted cardiomyocyte hypertrophy induced by such factors. These results uncover a potentially novel mechanism of CKD-induced LVH and provide a potential therapeutic target for CKD patients with LVH.

Keywords: Cardiology; Cardiovascular disease; Chronic kidney disease; Nephrology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Hypertrophy, Left Ventricular* / genetics
  • Hypertrophy, Left Ventricular* / metabolism
  • Hypertrophy, Left Ventricular* / pathology
  • Male
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Nephrectomy
  • Rats
  • Rats, Sprague-Dawley
  • Renal Insufficiency, Chronic* / genetics
  • Renal Insufficiency, Chronic* / metabolism
  • Renal Insufficiency, Chronic* / pathology

Substances

  • MIRN30 microRNA, rat
  • MicroRNAs