COVID-19 Severity Is Associated with Differential Antibody Fc-Mediated Innate Immune Functions

mBio. 2021 Apr 20;12(2):e00281-21. doi: 10.1128/mBio.00281-21.

Abstract

Beyond neutralization, antibodies binding to their Fc receptors elicit several innate immune functions including antibody-dependent complement deposition (ADCD), antibody-dependent cell-mediated phagocytosis (ADCP), and antibody-dependent cell-mediated cytotoxicity (ADCC). These functions are beneficial, as they contribute to pathogen clearance; however, they also can induce inflammation. We tested the possibility that qualitative differences in SARS-CoV-2-specific antibody-mediated innate immune functions contribute to coronavirus disease 2019 (COVID-19) severity. We found that anti-S1 and anti-RBD antibodies from hospitalized COVID-19 patients elicited higher ADCD but lower ADCP compared to antibodies from nonhospitalized COVID-19 patients. Consistently, higher ADCD was associated with higher systemic inflammation, whereas higher ADCP was associated with lower systemic inflammation during COVID-19. Our study points to qualitative, differential features of anti-SARS-CoV-2 specific antibodies as potential contributors to COVID-19 severity. Understanding these qualitative features of natural and vaccine-induced antibodies will be important in achieving optimal efficacy and safety of SARS-CoV-2 vaccines and/or COVID-19 therapeutics.IMPORTANCE A state of hyperinflammation and increased complement activation has been associated with coronavirus disease 2019 (COVID-19) severity. However, the pathophysiological mechanisms that contribute to this phenomenon remain mostly unknown. Our data point to a qualitative, rather than quantitative, difference in SARS-CoV-2-specific antibodies' ability to elicit Fc-mediated innate immune functions as a potential contributor to COVID-19 severity and associated inflammation. These data highlight the need for further studies to understand these qualitative features and their potential contribution to COVID-19 severity. This understanding could be essential to develop antibody-based COVID-19 therapeutics and SARS-CoV-2 vaccines with an optimal balance between efficacy and safety.

Keywords: COVID-19; Fc-mediated functions; SARS-CoV-2; antibody; inflammation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Neutralizing / blood
  • Antibodies, Viral* / blood
  • Antibodies, Viral* / immunology
  • Antibody Specificity
  • Antibody-Dependent Cell Cytotoxicity
  • Biomarkers / blood
  • COVID-19 / etiology
  • COVID-19 / immunology*
  • COVID-19 / virology
  • Case-Control Studies
  • Cohort Studies
  • Complement Activation
  • Female
  • Humans
  • Immunity, Innate*
  • Immunoglobulin Fc Fragments / immunology
  • Inflammation / blood
  • Inflammation / etiology
  • Inflammation / immunology
  • Male
  • Middle Aged
  • Pandemics
  • Phagocytosis
  • Receptors, Fc / immunology
  • SARS-CoV-2 / immunology*
  • Severity of Illness Index
  • Spike Glycoprotein, Coronavirus / immunology

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Biomarkers
  • Immunoglobulin Fc Fragments
  • Receptors, Fc
  • Spike Glycoprotein, Coronavirus
  • spike protein, SARS-CoV-2