Lipocalin-type Prostaglandin D Synthase Concentration Gradients in the Cerebrospinal Fluid in Normal-tension Glaucoma Patients with Optic Nerve Sheath Compartmentation

Achmed Pircher; Albert Neutzner; Margherita Montali; Andreas Huber; Hendrik P N Scholl; Jatta Berberat; Luca Remonda; Hanspeter E Killer

doi:10.2147/EB.S297274

Lipocalin-type Prostaglandin D Synthase Concentration Gradients in the Cerebrospinal Fluid in Normal-tension Glaucoma Patients with Optic Nerve Sheath Compartmentation

Eye Brain. 2021 Apr 14:13:89-97. doi: 10.2147/EB.S297274. eCollection 2021.

Authors

Achmed Pircher^{1

2}, Albert Neutzner³, Margherita Montali⁴, Andreas Huber⁵, Hendrik P N Scholl^{2

6}, Jatta Berberat⁷, Luca Remonda⁷, Hanspeter E Killer³

Affiliations

¹ Department of Neuroscience/Ophthalmology, Uppsala University, Uppsala, Sweden.
² Department of Ophthalmology, University Hospital Basel & University Basel, Basel, Switzerland.
³ Department of Biomedicine, University Hospital Basel & University Basel, Basel, Switzerland.
⁴ Department of Ophthalmology, San Bassiano Hospital, Bassano del Grappa, Italy.
⁵ Department of Medicine, Private University of the Principality of Lie Triesen, Triesen, Liechtenstein.
⁶ Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
⁷ Department of Neuroradiology, Cantonal Hospital Aarau, Aarau, Switzerland.

Abstract

Objective: To report on the lipocalin-type prostaglandin D synthase (L-PGDS) concentrations in the cerebrospinal fluid (CSF) of the perioptic and lumbar subarachnoid space (SAS) in patients with radiologically proven optic nerve (ON) sheath compartmentation presenting as normal-tension glaucoma (NTG).

Methods: Retrospective biochemical analysis of CSF in thirteen patients with ON sheath compartmentation presenting as NTG (four females, mean age 70±8 years). CSF was sampled from the SAS of the ON during ON sheath fenestration for ON sheath compartmentation and from the lumbar SAS at the time of lumbar puncture. Nephelometry was used for the quantification of L-PGDS and albumin concentration. Albumin was measured in order to assess the amount of contamination with serum in the CSF samples taken from the ON SAS. Main outcome measures were L-PGDS concentrations in the CSF of the perioptic and lumbar SAS.

Results: Mean L-PGDS concentration was 24±8 mg/L in the lumbar SAS compared to 33±27 mg/L without correction of serum contamination and 45±39 mg/L after correction of serum contamination in the perioptic SAS. The difference between the lumbar and the perioptic SAS was statistically significant (P=0.0047 without correction of serum contamination, P=0.0002 with correction of serum contamination; Mann-Witney U-test).

Conclusion: This study demonstrates a concentration gradient of L-PGDS levels within the CSF with a statistically significant higher concentration in the compartmentalized perioptic SAS compared to that in the lumbar SAS. Biochemical changes in the perioptic SAS might be involved in the pathophysiology in NTG patients with ON sheath compartmentation.

Keywords: cerebrospinal fluid; lipocalin-type prostaglandin D synthase; normal-tension glaucoma; optic nerve; optic nerve sheath compartment syndrome.