Genome and transcriptome of a pathogenic yeast, Candida nivariensis

Yunfan Fan; Andrew N Gale; Anna Bailey; Kali Barnes; Kiersten Colotti; Michal Mass; Luke B Morina; Bailey Robertson; Remy Schwab; Niki Tselepidakis; Winston Timp

doi:10.1093/g3journal/jkab137

Genome and transcriptome of a pathogenic yeast, Candida nivariensis

G3 (Bethesda). 2021 Jul 14;11(7):jkab137. doi: 10.1093/g3journal/jkab137.

Authors

Affiliations

¹ Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
² Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
³ Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
⁴ Division of Infectious Disease, Department of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.

Abstract

We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.

Keywords: Candida nivariensis; genome assembly; nanopore sequencing; pathogenic yeast; transcriptome.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Genome
RNA
Saccharomyces cerevisiae*
Sequence Analysis, DNA
Transcriptome*

Genome and transcriptome of a pathogenic yeast, Candida nivariensis

Authors

Affiliations

Abstract

Publication types

MeSH terms

Substances

Supplementary concepts

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