Shotgun Bacterial Lipid A Analysis Using Routine MALDI-TOF Mass Spectrometry

Methods Mol Biol. 2021:2306:275-283. doi: 10.1007/978-1-0716-1410-5_18.

Abstract

Detection of bacterial lipids and particularly the lipid A, the lipid anchor of the lipopolysaccharide, can be very challenging and requires a certain level of expertise. Here, this chapter describes a straightforward and simple method for the analysis of bacterial lipid A. In addition, such approach, lipid fingerprint, has the potential to be applied to other bacteria such as mycobacteria.

Keywords: Cell envelope; Gram-negative bacteria; Lipid A; MALDI-TOF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / chemistry*
  • Hydrolysis
  • Lipid A / analysis*
  • Lipidomics / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Workflow

Substances

  • Lipid A