Streptococcus vicugnae sp. nov., isolated from faeces of alpacas (Vicugna pacos) and cattle (Bos taurus), Streptococcus zalophi sp. nov., and Streptococcus pacificus sp. nov., isolated from respiratory tract of California sea lions (Zalophus californianus)

Abstract

Four novel independent strains of Streptococcus spp. were isolated from faeces of alpaca (SL1232^T), cattle (KCJ4950), and from respiratory tract of wild California sea lions (CSL7508^T, CSL7591^T). The strains were indole-, oxidase- and catalase-negative, non-spore-forming, non-motile Gram-positive cocci in short and long chains, facultative anaerobes. The 16S rRNA gene of SL1232^T and KCJ4950 shared 99.40-99.60% nucleotide similarity to strains of S. equinus, S. lutetiensis, S. infantarius, and the 16S rRNA gene of CSL7508^T and CSL7591^T demonstrated 98.72 and 98.92% similarity, respectively, to S. marimammalium. All other known Streptococcus species had the 16S rRNA gene sequence similarities of ≤95%. The genomes were sequenced for the novel strains. Average nucleotide identity (ANI) analysis for strains SL1232^T and KCJ4950, showed the highest similarity to S. equinus, S. lutetiensis, and S. infantarius with 85.21, 87.17, 88.47, 85.54, 87.47 and 88.89%, respectively, and strains CSL7508^T and CSL7591^T to S. marimammalium with 87.16 and 83.97%, respectively. Results of ANI were confirmed by pairwise digital DNA-DNA hybridization and phylogeny, which also revealed that the strains belong to three novel species of the genus Streptococcus. Phenotypical features of the novel species were in congruence with closely related members of the genus Streptococcus and gave negative reactions with the tested Lancefield serological groups (A-D, F and G). MALDI-TOF mass spectrometry supported identification of the species. Based on these data, we propose three novel species of the genus Streptococcus, for which the name Streptococcus vicugnae sp. nov. is proposed with the type strain SL1232^T (=NCTC 14341^T=DSM 110741^T=CCUG 74371^T), Streptococcus zalophi sp. nov. is proposed with the type strain CSL7508^T (=NCTC 14410^T=DSM 110742^T=CCUG 74374^T) and Streptococcus pacificus sp. nov. is proposed with the type strain CSL7591^T (=NCTC 14455^T=DSM 111148^T=CCUG 74655^T). The genome G+C content is 36.89, 34.85, and 35.34 % and draft genome sizes are 1906993, 1581094 and 1656080 bp for strains SL1232^T, CSL7508^T, and CSL7591^T, respectively.

Keywords: California sea lions; Streptococcus; alpacas; cattle; faeces; phylogeny; respiratory tract.