Antimicrobial and self-crosslinking potential of experimentally developed dioctadecyldimethyl ammonium bromide and riboflavin dentin adhesive

J Biomed Mater Res A. 2021 Nov;109(11):2392-2406. doi: 10.1002/jbm.a.37221. Epub 2021 May 21.

Abstract

The aim of the study is to investigate a new formulation, based on dioctadecyldimethyl ammonium-bromide (QA) and riboflavin (RF), combining antimicrobial activities and protease inhibitory properties with collagen crosslinking without interference to bonding capabilities in a rabbit model. Quaternary ammonium riboflavin (QARF) experimental adhesives modified with dioctadecyldimethyl ammonium-bromide and riboflavin were bonded (0.5/1.0/2.0%) to rabbit dentin to investigate for pulpal-histology, interfacial-morphology, transmission electron microscopy, mechanical properties, collagen crosslinking, micro-Raman analysis, antimicrobial, and anti-protease activities. Collagen type-I molecules were generated using molecular-docking. Odontoblasts appeared with normal histology, were seen in controls with no inflammatory cells detected in 0.5% specimens at day 7 and mild inflammatory response at day 30. In QARF 2.0%, inflammatory cells were not detected at day 7 and 30 (p < .05). Dentinal tubules are seen with intact collagen surface in 1% specimens. Resin penetrated inside 1% adhesive specimens with few irregularly funnel-shaped tags. Transmission electron microscopy showed thinner collagen in all specimens except 1% QARF specimens. Biofilms were influenced by QARF adhesives. Elastic moduli were significantly higher in 1.0% and 2.0% QARF adhesive specimens with a significant increase in total crosslinks. Stable amide groups with anti-protease activity was observed in QARF groups. Charged residues were seen in the triple helix hCOL3A1, Gly489-Gly510 after stabilisation with formulation. The 1% QARF modified adhesives improved biochemical and biomechanical properties of rabbit dentin.

Keywords: MMPs; Raman; adhesion; antimicrobial; collagen; crosslinking; rabbit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Infective Agents* / chemistry
  • Anti-Infective Agents* / pharmacology
  • Cross-Linking Reagents* / chemistry
  • Cross-Linking Reagents* / pharmacology
  • Dental Cements* / chemistry
  • Dental Cements* / pharmacology
  • Female
  • Male
  • Materials Testing*
  • Odontoblasts / metabolism*
  • Quaternary Ammonium Compounds* / chemistry
  • Quaternary Ammonium Compounds* / pharmacology
  • Rabbits
  • Riboflavin* / chemistry
  • Riboflavin* / pharmacology

Substances

  • Anti-Infective Agents
  • Cross-Linking Reagents
  • Dental Cements
  • Quaternary Ammonium Compounds
  • dimethyldioctadecylammonium
  • Riboflavin