A robust protocol for the generation of human midbrain organoids

Alise Zagare; Matthieu Gobin; Anna S Monzel; Jens C Schwamborn

doi:10.1016/j.xpro.2021.100524

A robust protocol for the generation of human midbrain organoids

STAR Protoc. 2021 May 4;2(2):100524. doi: 10.1016/j.xpro.2021.100524. eCollection 2021 Jun 18.

Authors

Alise Zagare¹, Matthieu Gobin¹, Anna S Monzel¹, Jens C Schwamborn¹

Affiliation

¹ Developmental and Cellular Biology, Luxembourg Centre for Systems Biomedecine (LCSB), University of Luxembourg, 6, Avenue du Swing, 4367 Belvaux, Luxembourg.

Abstract

The lack of advanced in vitro models recapitulating the human brain complexity is still a major obstacle in brain development and neurological disease research. Here, we describe a robust protocol to derive human midbrain organoids from neuroepithelial stem cells. These complex 3D models are characterized by the presence of functional neurons, including dopaminergic neurons and glial cells, making them particularly attractive for the study of Parkinson disease. For complete details on the use and execution of this protocol, please refer to Monzel et al. (2017).

Keywords: Cell Differentiation; Cell culture; Neuroscience; Organoids; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / methods*
Cells, Cultured
Humans
Mesencephalon / cytology*
Models, Neurological
Neural Stem Cells / cytology
Organoids / cytology*
Parkinson Disease