The FlhA linker mediates flagellar protein export switching during flagellar assembly

Commun Biol. 2021 May 31;4(1):646. doi: 10.1038/s42003-021-02177-z.

Abstract

The flagellar protein export apparatus switches substrate specificity from hook-type to filament-type upon hook assembly completion, thereby initiating filament assembly at the hook tip. The C-terminal cytoplasmic domain of FlhA (FlhAC) serves as a docking platform for flagellar chaperones in complex with their cognate filament-type substrates. Interactions of the flexible linker of FlhA (FlhAL) with its nearest FlhAC subunit in the FlhAC ring is required for the substrate specificity switching. To address how FlhAL brings the order to flagellar assembly, we analyzed the flhA(E351A/W354A/D356A) ΔflgM mutant and found that this triple mutation in FlhAL increased the secretion level of hook protein by 5-fold, thereby increasing hook length. The crystal structure of FlhAC(E351A/D356A) showed that FlhAL bound to the chaperone-binding site of its neighboring subunit. We propose that the interaction of FlhAL with the chaperon-binding site of FlhAC suppresses filament-type protein export and facilitates hook-type protein export during hook assembly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Bacterial Proteins / ultrastructure
  • Binding Sites
  • Flagella / metabolism*
  • Flagella / physiology
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Membrane Proteins / ultrastructure
  • Molecular Chaperones / genetics
  • Mutation / genetics
  • Protein Binding
  • Protein Transport / genetics
  • Salmonella enterica / metabolism*
  • Substrate Specificity

Substances

  • Bacterial Proteins
  • FlhA protein, Bacteria
  • Membrane Proteins
  • Molecular Chaperones