Multivariate optimization of a goat meat alkaline solubilization procedure using tetramethylammonium hydroxide for metals determination using FAAS

Juscelia Pereira Santos Alves; Uillian Mozart Ferreira da Mata Cerqueira; Cleber Galvão Novaes; Walter Nei Lopes Dos Santos; Sulene Alves Araújo; Marcos Almeida Bezerra

doi:10.1016/j.foodchem.2021.130176

Multivariate optimization of a goat meat alkaline solubilization procedure using tetramethylammonium hydroxide for metals determination using FAAS

Food Chem. 2021 Nov 15:362:130176. doi: 10.1016/j.foodchem.2021.130176. Epub 2021 Jun 2.

Authors

Juscelia Pereira Santos Alves¹, Uillian Mozart Ferreira da Mata Cerqueira², Cleber Galvão Novaes¹, Walter Nei Lopes Dos Santos³, Sulene Alves Araújo¹, Marcos Almeida Bezerra⁴

Affiliations

¹ Universidade Estadual do Sudoeste da Bahia, Campus de Jequié, Departamento de Química e Exatas, Rua José Moreira Sobrinho s/n, 45.206-190 Jequié, Bahia, Brazil.
² Universidade Federal da Bahia, Instituto de Química, Campus da Federação/Ondina, Rua Barão de Geremoabo s/n, 40.170-115 Salvador, Bahia, Brazil.
³ Universidade do Estado da Bahia, Departamento de Ciências Exatas e da Terra, Rua Silveira Martins, 2555, 40170-290 Salvador, Bahia, Brazil.
⁴ Universidade Estadual do Sudoeste da Bahia, Campus de Jequié, Departamento de Química e Exatas, Rua José Moreira Sobrinho s/n, 45.206-190 Jequié, Bahia, Brazil. Electronic address: mbezerra@uesb.edu.br.

PMID: 34111692
DOI: 10.1016/j.foodchem.2021.130176

Abstract

In the present work, multivariate designs were used to optimize an alkaline dissolution, assisted by ultrasound energy, procedure of goat meat using tetramethylammonium hydroxide (TMAH) aiming to determine Ca, Cu, Fe, K, Mg, Na and Zn by flame atomic absorption (FAAS) and emission (FAES) spectrometry. The optimal conditions found for the dissolution were in the following ranges: 0.4-0.5 g for the sample mass, 12-15 min of sonication and using 700-1000 µL of 25% TMAH at a temperature of 50 °C. The obtained limits of quantification varied between 0.221 (Mg) and 7.60 (Ca) μg g^-1. Accuracy was assessed by comparing the results obtained by applying the proposed method with the digestion in an acid medium using a digesting block and by analyzing bovine liver certified reference material. The application of a t-test revealed that, at a 95% confidence level, there were no significant differences between the values obtained.

Keywords: Alkaline solubilization; Atomic spectrometry; Goat meat; Metals; Multivariate optimization; TMAH.

MeSH terms

Animals
Food Analysis / methods*
Food Analysis / statistics & numerical data
Goats
Meat / analysis*
Metals / analysis*
Multivariate Analysis
Quaternary Ammonium Compounds / chemistry
Solubility
Spectrophotometry, Atomic / methods*
Spectrophotometry, Atomic / statistics & numerical data
Temperature

Substances

Metals
Quaternary Ammonium Compounds
tetramethylammonium