Optimized protocol for immunophenotyping of melanoma and tumor-bearing skin from mouse

Sai Sakktee Krisna; Christophe Goncalves; Natascha Gagnon; Fan Huang; Dany Plourde; Wilson H Miller Jr; Jörg H Fritz; Sonia V Del Rincon

doi:10.1016/j.xpro.2021.100627

Optimized protocol for immunophenotyping of melanoma and tumor-bearing skin from mouse

STAR Protoc. 2021 Jul 2;2(3):100627. doi: 10.1016/j.xpro.2021.100627. eCollection 2021 Sep 17.

Authors

Sai Sakktee Krisna^{1

2

3}, Christophe Goncalves³, Natascha Gagnon³, Fan Huang^{3

4}, Dany Plourde³, Wilson H Miller Jr^{3

4

5}, Jörg H Fritz^{1

2

6}, Sonia V Del Rincon^{3

4

5}

Affiliations

¹ Department of Physiology, Faculty of Medicine, McGill University, Montreal, QC H3G 1Y6, Canada.
² McGill University Research Centre on Complex Traits, Faculty of Medicine, Bellini Pavilion, McGill University, Montreal, QC H3G 0B1, Canada.
³ Segal Cancer Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC H3T 1E2, Canada.
⁴ Divsion of Experimental Medicine, Department of Medicine, McGill University, Montreal, QC H4A 3J1, Canada.
⁵ Department of Oncology, Faculty of Medicine, McGill University, Montreal, QC H4A 3T2, Canada.
⁶ Department of Microbiology and Immunology, Faculty of Medicine, McGill University, Montreal, QC H3A 2B4, Canada.

Abstract

While isolating immune cells from spleens and lungs is routinely achieved using flow cytometry, it is challenging to isolate viable immune cells from skin. Here, we describe a step-by-step protocol for skin digestion using a murine melanoma model, which is amenable for detection of low abundant immune cell populations including group 2 innate lymphoid cells.

Keywords: Cancer; Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Flow Cytometry / methods
Immunophenotyping*
Melanoma, Experimental / immunology*
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Transgenic
Skin / immunology*
Skin Neoplasms / immunology*

Grants and funding

PJT-162260 /CIHR/Canada