Amorphous-Crystalline Calcium Phosphate Coating Promotes In Vitro Growth of Tumor-Derived Jurkat T Cells Activated by Anti-CD2/CD3/CD28 Antibodies

Yurii P Sharkeev; Ekaterina G Komarova; Valentina V Chebodaeva; Mariya B Sedelnikova; Aleksandr M Zakharenko; Kirill S Golokhvast; Larisa S Litvinova; Olga G Khaziakhmatova; Vladimir V Malashchenko; Kristina A Yurova; Natalia D Gazatova; Ivan G Kozlov; Marina Y Khlusova; Konstantin V Zaitsev; Igor A Khlusov

doi:10.3390/ma14133693

Amorphous-Crystalline Calcium Phosphate Coating Promotes In Vitro Growth of Tumor-Derived Jurkat T Cells Activated by Anti-CD2/CD3/CD28 Antibodies

Materials (Basel). 2021 Jul 1;14(13):3693. doi: 10.3390/ma14133693.

Authors

Yurii P Sharkeev^{1

2}, Ekaterina G Komarova¹, Valentina V Chebodaeva¹, Mariya B Sedelnikova¹, Aleksandr M Zakharenko³, Kirill S Golokhvast³, Larisa S Litvinova⁴, Olga G Khaziakhmatova⁴, Vladimir V Malashchenko⁴, Kristina A Yurova⁴, Natalia D Gazatova⁴, Ivan G Kozlov⁵, Marina Y Khlusova⁶, Konstantin V Zaitsev⁷, Igor A Khlusov^{4

8

9}

Affiliations

¹ Laboratory of Physics of Nanostructured Biocomposites, Institute of Strength Physics and Materials Science, Siberian Branch of Russian Academy of Sciences, 634055 Tomsk, Russia.
² Research School of High-Energy Physics, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.
³ School of Engineering, Far Eastern Federal University, 690090 Vladivostok, Russia.
⁴ Center for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, 236029 Kaliningrad, Russia.
⁵ Department of Organization and Management in the Sphere of Circulation of Medicines, Institute of Postgraduate Education, I.M. Sechenov Federal State Autonomous Educational University of Higher Education-First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), 119991 Moscow, Russia.
⁶ Department of Pathophysiology, Siberian State Medical University, 634050 Tomsk, Russia.
⁷ Siberian Federal Scientific and Clinical Center of the Federal Medical-Biological Agency, 636070 Seversk, Russia.
⁸ Research School of Chemistry and Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.
⁹ Department of Morphology and General Pathology, Siberian State Medical University, 634050 Tomsk, Russia.

Abstract

A modern trend in traumatology, orthopedics, and implantology is the development of materials and coatings with an amorphous-crystalline structure that exhibits excellent biocopatibility. The structure and physico-chemical and biological properties of calcium phosphate (CaP) coatings deposited on Ti plates using the micro-arc oxidation (MAO) method under different voltages (200, 250, and 300 V) were studied. Amorphous, nanocrystalline, and microcrystalline statesof CaHPO₄ and β-Ca₂P₂O₇ were observed in the coatings using TEM and XRD. The increase in MAO voltage resulted in augmentation of the surface roughness R_a from 2.5 to 6.5 µm, mass from 10 to 25 mg, thickness from 50 to 105 µm, and Ca/P ratio from 0.3 to 0.6. The electrical potential (EP) of the CaP coatings changed from -456 to -535 mV, while the zeta potential (ZP) decreased from -53 to -40 mV following an increase in the values of the MAO voltage. Numerous correlations of physical and chemical indices of CaP coatings were estimated. A decrease in the ZP magnitudes of CaP coatings deposited at 200-250 V was strongly associated with elevated hTERT expression in tumor-derived Jurkat T cells preliminarily activated with anti-CD2/CD3/CD28 antibodies and then contacted in vitro with CaP-coated samples for 14 days. In turn, in vitro survival of CD4⁺ subsets was enhanced, with proinflammatory cytokine secretion of activated Jurkat T cells. Thus, the applied MAO voltage allowed the regulation of the physicochemical properties of amorphous-crystalline CaP-coatings on Ti substrates to a certain extent. This method may be used as a technological mechanism to trigger the behavior of cells through contact with micro-arc CaP coatings. The possible role of negative ZP and Ca²⁺ as effectors of the biological effects of amorphous-crystalline CaP coatings is discussed. Micro-arc CaP coatings should be carefully tested to determine their suitability for use in patients with chronic lymphoid malignancies.

Keywords: Jurkat T cell culture; amorphous–crystalline structure; cytokine secretion; electrical potential; gene expression; micro-arc oxidation; microstructure; zeta potential.

Abstract

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