Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni2+ magnetic beads

Anal Biochem. 2021 Oct 15:631:114360. doi: 10.1016/j.ab.2021.114360. Epub 2021 Sep 3.

Abstract

To monitor the levels of protecting antibodies raised in the population in response to infection and/or to immunization with SARS-CoV-2, we need a technique that allows high throughput and low-cost quantitative analysis of human IgG antibodies reactive against viral antigens. Here we describe an ultra-fast, high throughput and inexpensive assay to detect SARS-CoV-2 seroconversion in humans. The assay is based on Ni2+ magnetic particles coated with His tagged SARS-CoV-2 antigens. A simple and inexpensive 96 well plate magnetic extraction/homogenization process is described which allows the simultaneous analysis of 96 samples and delivers results in 7 min with high accuracy.

Keywords: COVID-19; High throughput; Magnetic ELISA; Magnetic beads; SARS-CoV-2.

Publication types

  • Evaluation Study

MeSH terms

  • Antibodies, Viral / blood*
  • Antibodies, Viral / immunology
  • Antigens, Viral / blood
  • Antigens, Viral / immunology
  • COVID-19 / blood
  • COVID-19 / diagnosis*
  • COVID-19 / immunology
  • COVID-19 Serological Testing / economics
  • COVID-19 Serological Testing / methods*
  • Enzyme-Linked Immunosorbent Assay / economics
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Immunoglobulin G / blood*
  • Immunoglobulin G / immunology
  • Magnets / chemistry
  • Nickel / chemistry
  • SARS-CoV-2 / immunology
  • SARS-CoV-2 / isolation & purification*
  • Sensitivity and Specificity
  • Seroconversion
  • Time Factors

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Immunoglobulin G
  • Nickel