SNP rs657152 Is Not Associated with the Level of Viral Load in COVID-19 or the Probability of Disease in the Population of Caucasians in Eastern Siberia

E A Orlova; O B Ogarkov; P A Khromova; V V Sinkov; M A Khasnatinov; S N Zhdanova; L V Rychkova; L I Kolesnikova

doi:10.1134/S1022795421080093

SNP rs657152 Is Not Associated with the Level of Viral Load in COVID-19 or the Probability of Disease in the Population of Caucasians in Eastern Siberia

Russ J Genet. 2021;57(8):982-984. doi: 10.1134/S1022795421080093. Epub 2021 Aug 30.

Authors

E A Orlova¹, O B Ogarkov¹, P A Khromova¹, V V Sinkov¹, M A Khasnatinov¹, S N Zhdanova¹, L V Rychkova¹, L I Kolesnikova¹

Affiliation

¹ Scientific Center for Family Health and Human Reproduction Problems, 664003 Irkutsk, Russia.

Abstract

Cross-replicating associations with rs657152 at the 9q34.2c locus and rs11385942 at the 3p21.31 locus found in patients with severe COVID-19 in the Caucasian population require the study of the discovered phenomenon in various populations, including as an independent biological marker. Primers and TaqMan probes for PCR discrimination of the A and C alleles in single nucleotide polymorphism (SNP) rs657152 have been developed. The polymorphism of the rs657152 A/C locus was determined in 129 patients with COVID-19 and in a control group of 466 healthy individuals. There were no significant differences in the frequency of distribution of the A and C alleles, 0.47/0.53 and 0.45/0.55, between patients and healthy subjects, respectively. Also, no differences were found in the distribution of alleles in patients with a high viral load in the smear (Ct in the range of 16-25) in comparison with an average and low viral load (Ct in the range of 26-40).

Keywords: COVID-19; PCR; TaqMan probes; allele discrimination; rs657152.

© Pleiades Publishing, Inc. 2021, ISSN 1022-7954, Russian Journal of Genetics, 2021, Vol. 57, No. 8, pp. 982–984. © Pleiades Publishing, Inc., 2021.Russian Text © The Author(s), 2021, published in Genetika, 2021, Vol. 57, No. 8, pp. 974–976.