A Thrombectomy Model Based on Ex Vivo Whole Human Brains

AJNR Am J Neuroradiol. 2021 Nov;42(11):1968-1972. doi: 10.3174/ajnr.A7291. Epub 2021 Sep 23.

Abstract

Background and purpose: The persistent challenges in thrombectomy for large-vessel occlusion, such as suboptimal complete recanalization and first-pass effect imply an insufficient understanding of the artery-clot-device interaction. In this study, we present a thrombectomy model using fresh human brains, which can capture the artery-clot-device interaction through concurrent transmural and angiographic visualizations.

Materials and methods: Fresh nonfrozen whole adult human brains were collected and connected to a customized pump system tuned to deliver saline flow at a physiologic flow rate and pressure. Angiography was performed to verify the flow in the anterior-posterior and vertebrobasilar circulations and collaterals. Large-vessel occlusion was simulated by embolizing a radiopaque clot analog. Thrombectomy was tested, and the artery-clot-device interactions were recorded by transmural and angiographic videos.

Results: Baseline cerebral angiography revealed excellent penetration of contrast in the anterior-posterior and vertebrobasilar circulations without notable arterial cutoffs and with robust collaterals. Small branches (<0.5 mm) and perforating arteries were consistently opacified with good patency. Three device passes were performed to achieve recanalization, with failure modes including elongation, fragmentation, and distal embolization.

Conclusions: This model enables concurrent transmural and angiographic analysis of artery-clot-device interaction in a human brain and provides critical insights into the action mechanism and failure modes of current and upcoming thrombectomy devices.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Brain / diagnostic imaging
  • Brain / surgery
  • Embolization, Therapeutic*
  • Humans
  • Stroke* / therapy
  • Thrombectomy
  • Thrombosis*
  • Treatment Outcome