The main microbial contaminants of rooms in which laboratory rodents are housed are bacteria and fungi. Restriction of microbial growth to below threshold levels requires the application of various sophisticated antimicrobial techniques that must be effective and safe for the animals. Some of the most commonly used techniques, including chemical disinfection, ventilation, filtration, sterilization and radiation, are not always sufficiently effective. The aim of the current study was to evaluate the efficacy of a modern technique (i.e. radiant catalytic ionization (RCI)) on the microbiological status of an animal care facility, and the health of the mice housed therein. The experiment, conducted over seven days, compared an experimental room with an RCI system permanently turned on with a negative control room. At the completion of the experiment, the number of bacteria in the RCI room air and on its walls was lower than that in the control room (p < 0.01 in both cases). Values of the basic prooxidative parameter, thiobarbituric acid reactive substances concentration, in tissues of mice from the RCI room were within allowed boundaries. Hence, application of an RCI system proved to be an ideal technique to ensure high hygienic standards in animal rooms without any adverse effects on the animals housed therein.
Keywords: Radiant catalytic ionization; TBARS; animal facilities; mice; microorganisms.