Polyphenolic extracts from pomegranate and watermelon wastes as substrate to fabricate sustainable silver nanoparticles with larvicidal effect against Spodoptera littoralis

Ahmed M Saad; Mohamed T El-Saadony; Amira M El-Tahan; Samy Sayed; Moataz A M Moustafa; Ayman E Taha; Taha F Taha; Mahmoud M Ramadan

doi:10.1016/j.sjbs.2021.06.011

Polyphenolic extracts from pomegranate and watermelon wastes as substrate to fabricate sustainable silver nanoparticles with larvicidal effect against Spodoptera littoralis

Saudi J Biol Sci. 2021 Oct;28(10):5674-5683. doi: 10.1016/j.sjbs.2021.06.011. Epub 2021 Jun 10.

Authors

Ahmed M Saad¹, Mohamed T El-Saadony², Amira M El-Tahan³, Samy Sayed⁴, Moataz A M Moustafa⁵, Ayman E Taha⁶, Taha F Taha¹, Mahmoud M Ramadan⁷

Affiliations

¹ Biochemistry Department, Faculty of Agriculture Zagazig University, Zagazig 44511, Egypt.
² Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt.
³ Plant Production Department, Arid Lands Cultivation Research Institute, The City of Scientific Research and Technological Applications, SRTA-City. Borg El Arab, Alexandria, Egypt.
⁴ Department of Science and Technology, University College-Ranyah, Taif University, B.O. Box 11099, Taif 21944, Saudi Arabia.
⁵ Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt.
⁶ Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Edfina, 22578, Egypt.
⁷ Plant Protection Department, Faculty of Agriculture, Zagazig University, Egypt.

Abstract

The agricultural wastes adversely affect the environment; however, they are rich in polyphenols; therefore, this study aimed to employ polyphenol-enriched waste extracts for silver nanoparticles synthesis, and study the larvicidal activity of silver nanoparticles fabricated by pomegranate and watermelon peels extracts (PPAgNPs and WPAgNPs) against all larval instars of Spodoptera littoralis. The polyphenol profile of pomegranate and watermelon peel extracts (PP and WP) and silver nanoparticles was detected by HPLC. The antioxidant activity was estimated by DPPH, and FARP assays and the antimicrobial activity was evaluated by disc assay. The Larvicidal activity of AgNPs against Egyptian leaf worm was performed by dipping technique. The obtained AgNPs were spherical with size ranged 15-85 nm and capped with proteins and polyphenols. The phenolic compounds in silver nanoparticles increased about extracts; therefore, they have the best performance in antioxidant/reducing activity, and inhibit the growth of tested bacteria and yeast. The PPAgNPs were the most effective against the first instar larvae instar (LC₅₀ = 68.32 µg/ml), followed by pomegranate extract with (LC₅₀ = 2852 µg/ml). The results indicated that obvious increase in polyphenols content in silver nanoparticles enhance their larvicidal effect and increasing mortality of 1^st larval of S. littoralis Egyptian leafworms causing additive effect and synergism. We recommend recycling phenolic enriched agricultural wastes in producing green silver nanoprticles to control cotton leafworm that causes economic loses to crops.

Keywords: Antimicrobial; Antioxidant; Fruit peels; Larvicidal; Polyphenols; Silver nanoparticles.