Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution

Mengmeng Jiang; Yanyu Xiao; Weigao E; Lifeng Ma; Jingjing Wang; Haide Chen; Ce Gao; Yuan Liao; Qile Guo; Jinrong Peng; Xiaoping Han; Guoji Guo

doi:10.3389/fcell.2021.743421

Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution

Front Cell Dev Biol. 2021 Oct 1:9:743421. doi: 10.3389/fcell.2021.743421. eCollection 2021.

Authors

Mengmeng Jiang^{1

2

3}, Yanyu Xiao³, Weigao E³, Lifeng Ma³, Jingjing Wang^{1

2

3}, Haide Chen^{1

2

3}, Ce Gao⁴, Yuan Liao³, Qile Guo⁵, Jinrong Peng⁴, Xiaoping Han^{1

3}, Guoji Guo^{1

2

3}

Affiliations

¹ Bone Marrow Transplantation Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
² Liangzhu Laboratory, Zhejiang University Medical Center, Hangzhou, China.
³ Center for Stem Cell and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, China.
⁴ College of Animal Sciences, Zhejiang University, Hangzhou, China.
⁵ ZJU-UOE Institute, Zhejiang University School of Medicine, Haining, China.

Abstract

Zebrafish have been found to be a premier model organism in biological and regeneration research. However, the comprehensive cell compositions and molecular dynamics during tissue regeneration in zebrafish remain poorly understood. Here, we utilized Microwell-seq to analyze more than 250,000 single cells covering major zebrafish cell types and constructed a systematic zebrafish cell landscape. We revealed single-cell compositions for 18 zebrafish tissue types covering both embryo and adult stages. Single-cell mapping of caudal fin regeneration revealed a unique characteristic of blastema population and key genetic regulation involved in zebrafish tissue repair. Overall, our single-cell datasets demonstrate the utility of zebrafish cell landscape resources in various fields of biological research.

Keywords: cross-species analysis; regeneration; sequencing; single cell; zebrafish.