Proximity labeling: an enzymatic tool for spatial biology

Chang-Ryul Choi; Hyun-Woo Rhee

doi:10.1016/j.tibtech.2021.09.008

Proximity labeling: an enzymatic tool for spatial biology

Trends Biotechnol. 2022 Feb;40(2):145-148. doi: 10.1016/j.tibtech.2021.09.008. Epub 2021 Oct 16.

Authors

Chang-Ryul Choi¹, Hyun-Woo Rhee²

Affiliations

¹ Department of Chemistry, Seoul National University, Seoul 08826, Korea.
² Department of Chemistry, Seoul National University, Seoul 08826, Korea; School of Biological Sciences, Seoul National University, Seoul 08826, Korea. Electronic address: rheehw@snu.ac.kr.

PMID: 34663510
DOI: 10.1016/j.tibtech.2021.09.008

Abstract

In this Forum, we highlight how cutting-edge, proximity-dependent, enzymatic labeling tools, aided by sequencing technology developments, have enabled the extraction of spatial information of proteomes, transcriptomes, genome organization, and cellular networks. We also discuss the potential applications of proximity labeling in the unexplored field of spatial biology in live systems.

Keywords: APEX; TurboID; cell–cell interaction network; proximity labeling; spatial biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biology*
Proteome*

Substances

Proteome