Association of HER1 and HER2 Gene Variants in the Predisposition of Colorectal Cancer

Ibrahim O Alanazi; Jilani Purusottapatnam Shaik; Narasimha Reddy Parine; Nahla A Azzam; Othman Alharbi; Yousef M Hawsawi; Atif Abdulwahab A Oyouni; Osama M Al-Amer; Faisal Alzahrani; Majid A Almadi; Abdulrahman M Aljebreen; Mohammad Saud Alanazi; Zahid Khan

doi:10.1155/2021/6180337

Association of HER1 and HER2 Gene Variants in the Predisposition of Colorectal Cancer

J Oncol. 2021 Oct 21:2021:6180337. doi: 10.1155/2021/6180337. eCollection 2021.

Authors

Ibrahim O Alanazi^{1

2}, Jilani Purusottapatnam Shaik², Narasimha Reddy Parine², Nahla A Azzam^{3

4}, Othman Alharbi^{3

4}, Yousef M Hawsawi^{5

6}, Atif Abdulwahab A Oyouni^{7

8}, Osama M Al-Amer^{8

9}, Faisal Alzahrani¹⁰, Majid A Almadi^{3

4}, Abdulrahman M Aljebreen^{3

4}, Mohammad Saud Alanazi², Zahid Khan²

Affiliations

¹ The National Center for Biotechnology, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi Arabia.
² Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
³ College of Medicine, King Saud University, Riyadh, Saudi Arabia.
⁴ Division of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia.
⁵ Research Center, King Faisal Specialist Hospital and Research Center, MBC-J04, PO Box 40047, Jeddah 21499, Saudi Arabia.
⁶ College of Medicine, Alfaisal University, P.O. Box 50927, Riyadh 11533, Saudi Arabia.
⁷ Department of Biology, Faculty of Sciences, University of Tabuk, Tabuk, Saudi Arabia.
⁸ Genome and Biotechnology Unit, Faculty of Sciences, University of Tabuk, Tabuk, Saudi Arabia.
⁹ Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia.
¹⁰ Department of Biochemistry, Faculty of Science, Embryonic Stem Cells Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

Abstract

Background: Colorectal cancer (CRC) is a major health concern worldwide. A series of sequential accumulation of genetic and epigenetic changes are responsible for the initiation and progression of diseases via the normal > adenoma > carcinoma sequence. Genetic variants in crucial cancer-causing genes are known to mediate the risk of cancer.

Objective: In this case-control study, we examined single nucleotide polymorphism (SNP) in HER1 (rs763317 and rs3752651) and HER2 (rs1136201 and rs1058808) genes to assess their role in the susceptibility of CRC in a Saudi population.

Methods: TaqMan allelic discrimination assay was utilized to identify the genotypes in 163 normal and 143 CRC patients.

Results: In the overall analysis, the rs3752651 and rs1136201 were significantly associated with the risk of CRC. Although none of the examined SNPs had any impact on the age at which CRC was diagnosed, interestingly, three SNPs showed a significant association based on gender. The rs3752651 conferred significant protection only in men, whereas rs1136201 diminished the risk and rs1058808 considerably increased the susceptibility of CRC only in women.

Conclusions: Our result suggests that these SNPs in HER1 and HER2 after validation in larger cohorts of different ethnicities may be utilized as genetic screening markers for predicting colorectal cancer predisposition.