Effects of pyridinium-based ionic liquids with different alkyl chain lengths on the growth of maize seedlings

Abstract

The effects of four water-soluble pyridinium-based ionic liquids (ILs) differing in alkyl side chain length, namely, N-ethyl pyridinium bromide ([EPy]Br), N-butyl pyridinium bromide ([BPy]Br), N-hexyl pyridinium bromide ([HPy]Br), and N-octyl pyridinium bromide ([OPy]Br), on the growth of maize seedlings were investigated for the first time. The results revealed that the phytotoxicity of these ILs was significantly correlated with their concentration and alkyl side chain length. The 8-day 50% inhibition values indicated that the toxicity increased as the length of the alkyl chain increased: [EPy]Br < [BPy]Br < [HPy]Br < [OPy]Br. In addition, root growth was found to be more sensitive to ILs than stem growth. In response to exposure to ILs of increasing concentration, we observed different trends in the pigment contents and specific antioxidant enzyme activities in maize seedlings, whereas the contents of malondialdehyde were significantly increased. In addition, RNA sequencing analysis, performed to examine the gene expression profiles of maize leaves under [HPy]Br and [OPy]Br treatments, revealed that a larger number of genes were differentially expressed in response to [OPy]Br treatment. Furthermore, pathway enrichment analysis revealed that both [HPy]Br and [OPy]Br treatments, and particularly the latter, caused a down-regulation of genes involved in photosynthesis and carbohydrate and nitrogen metabolism. Our findings thus indicate that pyridinium-based IL toxicity might be associated with oxidative stress and changes in gene expression profiles.

Keywords: Maize; Metabolism; Oxidative damage; Pyridinium-based ionic liquids; RNA sequencing.