De novo transcriptome assembly and annotation of parthenogenetic lizard Darevskia unisexualis and its parental ancestors Darevskia valentini and Darevskia raddei nairensis

Sergei S Ryakhovsky; Victoria A Dikaya; Vitaly I Korchagin; Andrey A Vergun; Lavrentii G Danilov; Sofia D Ochkalova; Anastasiya E Girnyk; Daria V Zhernakova; Marine S Arakelyan; Vladimir B Brukhin; Aleksey S Komissarov; Alexey P Ryskov

doi:10.1016/j.dib.2021.107685

De novo transcriptome assembly and annotation of parthenogenetic lizard Darevskia unisexualis and its parental ancestors Darevskia valentini and Darevskia raddei nairensis

Data Brief. 2021 Dec 6:39:107685. doi: 10.1016/j.dib.2021.107685. eCollection 2021 Dec.

Authors

Sergei S Ryakhovsky^{1

2}, Victoria A Dikaya², Vitaly I Korchagin¹, Andrey A Vergun^{1

3}, Lavrentii G Danilov⁴, Sofia D Ochkalova^{1

2}, Anastasiya E Girnyk¹, Daria V Zhernakova^{1

5}, Marine S Arakelyan⁶, Vladimir B Brukhin⁷, Aleksey S Komissarov^{1

2}, Alexey P Ryskov¹

Affiliations

¹ Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow 119334, Russia.
² Applied Genomics Laboratory, SCAMT Institute, ITMO University, Lomonosova 9 Str., Saint Petersburg 197101, Russia.
³ Department of Biochemistry, Molecular biology and Genetics, Moscow Pedagogical State University, 1/1 M.Pirogovskaya Str., Moscow 119991, Russia.
⁴ Department of Genetics and Biotechnology, St. Petersburg State University, 7/9 Universitetskaya Nab., St. Petersburg 199034, Russia.
⁵ Laboratory of Genomic Diversity, Center for Computer Technologies, ITMO University, Kronverksky Ave. 49, St. Petersburg 197101, Russia.
⁶ Faculty of Biology, Yerevan State University, 1 Alex Manoogian, 0025, Yerevan , Armenia.
⁷ Plant Genomics Laboratory, SCAMT Institute, ITMO University, Saint Petersburg 197101, Russia.

Abstract

Darevskia rock lizards include 29 sexual and seven parthenogenetic species of hybrid origin distributed in the Caucasus. All seven parthenogenetic species of the genus Darevskia were formed as a result of interspecific hybridization of only four sexual species. It remains unknown what are the main advantages of interspecific hybridization along with switching on parthenogenetic reproduction in evolution of reptiles. Data on whole transcriptome sequencing of parthenogens and their parental ancestors can provide value impact in solving this problem. Here we have sequenced ovary tissue transcriptomes from unisexual parthenogenetic lizard D. unisexualis and its parental bisexual ancestors to facilitate the subsequent annotation and to obtain the collinear characteristics for comparison with other lizard species. Here we report generated RNAseq data from total mRNA of ovary tissues of D. unisexualis, D. valentini and D. raddei with 58932755, 51634041 and 62788216 reads. Obtained RNA reads were assembled by Trinity assembler and 95141, 62123, 61836 contigs were identified with N50 values of 2409, 2801 and 2827 respectively. For further analysis top Gene Ontology terms were annotated for all species and transcript number was calculated. The raw data were deposited in the NCBI SRA database (BioProject PRJNA773939). The assemblies are available in Mendeley Data and can be accessed via doi:10.17632/rtd8cx7zc3.1.

Keywords: AMP; Darevskia lizards; Ovaries; Parthenogenesis; Transcriptome analysis.