Protocol for controlled cortical impact in human cerebral organoids to model traumatic brain injury

Santiago Ramirez; Abhisek Mukherjee; Sofia E Sepulveda; Camila Gherardelli; Andrea Becerra-Calixto; Nicolas Bravo-Vasquez; Claudio Soto

doi:10.1016/j.xpro.2021.100987

Protocol for controlled cortical impact in human cerebral organoids to model traumatic brain injury

STAR Protoc. 2021 Dec 4;2(4):100987. doi: 10.1016/j.xpro.2021.100987. eCollection 2021 Dec 17.

Authors

Santiago Ramirez¹, Abhisek Mukherjee¹, Sofia E Sepulveda¹, Camila Gherardelli¹, Andrea Becerra-Calixto¹, Nicolas Bravo-Vasquez¹, Claudio Soto¹

Affiliation

¹ Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology, McGovern Medical School, University of Texas Health Science at Houston, Houston, TX 77030, USA.

Abstract

Modeling traumatic brain injury (TBI) has been a challenge. Rodent and cellular models have provided relevant contributions despite their limitations. Here, we present a protocol for a TBI model based on the controlled cortical impact (CCI) performed on human cerebral organoids (COs), self-assembled 3D cultures that recapitulate features of the human brain. Here, we generate COs from iPSCs obtained from reprogrammed fibroblasts. For complete details on the use and execution of this protocol, please refer to Ramirez et al. (2021).

Keywords: Neuroscience; Organoids; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / physiology
Brain Injuries, Traumatic / physiopathology*
Cells, Cultured
Humans
Induced Pluripotent Stem Cells / cytology
Male
Mice
Models, Biological*
Organoids* / cytology
Organoids* / injuries
Organoids* / physiopathology
Skull / physiology